EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.1.2.2 | of mutant K166R | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.2.2 | K166E | K166R retains low level of racemase activity. K166R mutant catalyzes the elimination of Br- from only the (R)-enantiomer of (R,S)-p-(bromomethyl)mandelate | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.34 | - |
(R)-mandelate | wild type | Pseudomonas putida | |
5.1.2.2 | 0.35 | - |
(S)-Mandelate | wild type | Pseudomonas putida | |
5.1.2.2 | 0.59 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida | |
5.1.2.2 | 1.4 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.2.2 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.2.2 | wild type and mutant K166R | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.2.2 | D-mandelate | - |
Pseudomonas putida | L-mandelate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.46 | - |
(S)-Mandelate | mutant K166R | Pseudomonas putida | |
5.1.2.2 | 480 | - |
(R)-mandelate | wild type enzyme | Pseudomonas putida |