EC Number | General Stability | Organism |
---|---|---|
1.14.15.4 | deoxycorticosterone stabilizes | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | acetone | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
1.14.15.4 | antiserum | produced by rabbits | Bos taurus | |
1.14.15.4 | CO | - |
Bos taurus | |
1.14.15.4 | deoxycorticosterone | competitive substrate inhibition | Bos taurus | |
1.14.15.4 | ethanol | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
1.14.15.4 | KCl | total inactivation | Bos taurus | |
1.14.15.4 | methanol | 10%, v/v, complete inhibition of hydroxylation | Bos taurus | |
1.14.15.4 | Metyrapone | competitive substrate inhibition | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.4 | mitochondrion | - |
Bos taurus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.4 | additional information | Bos taurus | corticosterone normal substrate in vivo | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.4 | Bos taurus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.15.4 | a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | both hydroxylation activities seem to occur in a single enzyme | Bos taurus | |
1.14.15.4 | a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | heme protein of so-called P-450 family | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.4 | adrenal cortex | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 0.000171 | - |
18-hydroxylation activity, 4% O2, 96% N2 | Bos taurus |
1.14.15.4 | 0.000211 | - |
18-hydroxylation activity, 21% O2, 79% N2 | Bos taurus |
1.14.15.4 | 0.00205 | - |
11beta-hydroxylation activity, 4% O2, 96% N2 | Bos taurus |
1.14.15.4 | 0.00278 | - |
11beta-hydroxylation activity, 21% O2, 79% N2 | Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.15.4 | 4°C, 11 days, both activities decrease considerably in a similar way, deoxycorticosterone delays decline | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
1.14.15.4 | corticosterone + reduced adrenal ferredoxin + O2 | addition of adrenodoxin and adrenodoxin reductase | Bos taurus | 18-hydroxy-11-deoxycorticosterone + oxidized adrenal ferredoxin + ? | - |
? | |
1.14.15.4 | additional information | corticosterone normal substrate in vivo | Bos taurus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 20 | - |
- |
Bos taurus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 2 | 50 | at 50°C almost no activity | Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 2 | - |
14% residual activity of 11beta- and 18-hydroxylation | Bos taurus |
1.14.15.4 | 30 | - |
after preincubation for 20 min, 27% residual activity, after 60 min 5.7% | Bos taurus |
1.14.15.4 | 50 | - |
complete inactivation | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | NADPH | - |
Bos taurus |