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Literature summary extracted from

  • Yum, D-Y.; Bae, S-S.; Pan, J-G.
    Purification and characterization of the 2-ketoaldonate reductase from Brevibacterium ketosoreductum ATCC21914 (1998), Biosci. Biotechnol. Biochem., 62, 154-156.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.215 0.01
-
 NADPH
-
 Brevibacterium ketosoreductum
1.1.1.215 5
-
 2,5-diketo-D-gluconate
-
 Brevibacterium ketosoreductum
1.1.1.346 0.01
-
 NADPH at pH 6.4 and 30°C Brevibacterium ketosoreductum
1.1.1.346 5
-
 2,5-didehydro-D-gluconate at pH 6.4 and 30°C Brevibacterium ketosoreductum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 35000
-
 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum
1.1.1.215 72000
-
 gel filtration Brevibacterium ketosoreductum
1.1.1.346 35000
-
 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum
1.1.1.346 72000
-
 gel filtration Brevibacterium ketosoreductum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 2,5-diketo-D-gluconate + NADPH Brevibacterium ketosoreductum
-
 5-keto-D-gluconate + NADP+
-
 ir
1.1.1.215 2,5-diketo-D-gluconate + NADPH Brevibacterium ketosoreductum ATCC 21914
-
 5-keto-D-gluconate + NADP+
-
 ir
1.1.1.215 2-dehydro-D-gluconate + NADPH Brevibacterium ketosoreductum
-
 D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH Brevibacterium ketosoreductum ATCC 21914
-
 D-gluconate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Brevibacterium ketosoreductum
-
-
-
1.1.1.346 Brevibacterium ketosoreductum
-
-
-
1.1.1.346 Brevibacterium ketosoreductum ATCC 21914
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215 ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200 Brevibacterium ketosoreductum
1.1.1.346 ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration Brevibacterium ketosoreductum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 22.2
-
-
 Brevibacterium ketosoreductum
1.1.1.346 0.07
-
 crude extract, at pH 6.4 and 30°C Brevibacterium ketosoreductum
1.1.1.346 22.2
-
 after 317fold purification, at pH 6.4 and 30°C Brevibacterium ketosoreductum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2,5-diketo-D-gluconate + NADPH
-
 Brevibacterium ketosoreductum 5-keto-D-gluconate + NADP+
-
 ir
1.1.1.215 2,5-diketo-D-gluconate + NADPH
-
 Brevibacterium ketosoreductum ATCC 21914 5-keto-D-gluconate + NADP+
-
 ir
1.1.1.215 2-dehydro-D-gluconate + NADPH
-
 Brevibacterium ketosoreductum D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH 11.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH
-
 Brevibacterium ketosoreductum ATCC 21914 D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH 11.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum ATCC 21914 D-gluconate + NADP+
-
 ?
1.1.1.215 2-keto-L-gulonate + NADPH 10.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum L-idonate + NADP+
-
 ?
1.1.1.215 2-keto-L-gulonate + NADPH 10.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum ATCC 21914 L-idonate + NADP+
-
 ?
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+ 100% activity Brevibacterium ketosoreductum 5-dehydro-D-gluconate + NADP+
-
 ?
1.1.1.346 2-dehydro-D-gluconate + NADPH + H+ 11.8% activity compared to 2,5-didehydro-D-gluconate Brevibacterium ketosoreductum D-gluconate + NADP+
-
 ?
1.1.1.346 2-dehydro-L-gulonate + NADPH + H+ 10.8% activity compared to 2,5-didehydro-D-gluconate Brevibacterium ketosoreductum L-idonate + NADP+
-
 ?
1.1.1.346 additional information no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate Brevibacterium ketosoreductum ?
-
 ?
1.1.1.346 additional information no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate Brevibacterium ketosoreductum ATCC 21914 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 dimer 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum
1.1.1.346 homodimer 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.346 25DKG reductase
-
 Brevibacterium ketosoreductum
1.1.1.346 2KR
-
 Brevibacterium ketosoreductum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 6
-
-
 Brevibacterium ketosoreductum
1.1.1.346 6
-
-
 Brevibacterium ketosoreductum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.215 6 7.5 35% of activity left after incubation at pH 8.4 for 2 h at 30°C, no remaining activity below pH 5.2 or above pH 10.0 Brevibacterium ketosoreductum
1.1.1.346 5.2 10 after 2 h incubation at 30°C, the remaining activity is about 35% of the original level at pH 8.4, and no remaining activity is observed below pH 5.1 and 10.0. The enzyme is stable from pH 6.0 to 7.5 Brevibacterium ketosoreductum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADP+
-
 Brevibacterium ketosoreductum
1.1.1.215 NADPH
-
 Brevibacterium ketosoreductum
1.1.1.346 NADPH
-
 Brevibacterium ketosoreductum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.346 Brevibacterium ketosoreductum isoelectric focusing
-
 4.7