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Literature summary extracted from

  • Yum, D-Y.; Lee, B-Y.; Hahm, D-H.; Pan, J-G.
    The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase (1998), J. Bacteriol., 180, 5984-5988.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 expressed in Escherichia coli Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 36000
-
 2 * 36000, SDS-PAGE Escherichia coli
1.1.1.215 74000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 2,5-diketo-D-gluconate + NADPH Escherichia coli
-
 5-keto-D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH Escherichia coli
-
 D-gluconate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Escherichia coli
-
 constitutive expressed enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215 His6-tagged enzyme, metal-chelate affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2,5-diketo-D-gluconate + NADPH
-
 Escherichia coli 5-keto-D-gluconate + NADP+
-
 ?
1.1.1.215 2-dehydro-D-gluconate + NADPH
-
 Escherichia coli D-gluconate + NADP+
-
 ?
1.1.1.215 2-keto-L-gulonate + NADPH
-
 Escherichia coli L-idonate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 dimer 2 * 36000, SDS-PAGE Escherichia coli