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Literature summary extracted from

  • Beedle, A.S.; Rees, H.H.; Goodwin, T.W.
    Some properties and a suggested reclassification of mevaldate reductase (1974), Biochem. J., 139, 205-209.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.33 Sodium aminobarbitone 1 mM, 48% inhibition, noncompetitive Sus scrofa
1.1.1.33 Thiobarbituric acid 1 mM, 22% inhibition, noncompetitive Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.33 0.09
-
 NADPH
-
 Sus scrofa
1.1.1.33 0.15
-
 4-cyanobenzaldehyde
-
 Sus scrofa
1.1.1.33 0.26
-
 (3RS)-mevaldate
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.33 30000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.33 (R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH Sus scrofa
-
 (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.33 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.33 ammonium sulfate precipitation, calcium phosphate gel, gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.33 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.33 0.573
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.33 -20°C, 3 months, 0% loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.33 (3RS)-mevaldate + NADPH + H+
-
 Sus scrofa (R)-mevalonate + NADP+
-
 r
1.1.1.33 (R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
-
 Sus scrofa (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
 ir
1.1.1.33 (S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
 Sus scrofa (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir
1.1.1.33 4-cyanobenzaldehyde + NADPH
-
 Sus scrofa 4-cyanobenzylalcohol + NADP+
-
 ir
1.1.1.33 pyridine-3-carboxaldehyde + NADPH
-
 Sus scrofa pyridine-3-methanol + NADP+
-
 ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.33 NADH giving one-fifth of the rate obtained with NADPH Sus scrofa
1.1.1.33 NADPH
-
 Sus scrofa