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Literature summary extracted from

  • Ball, K.L.; Dale, S.; Weekes, J.; Hardie, D.G.
    Biochemical characterization of two forms of 3-hydroxy-3-methylglutaryl-CoA reductase kinase from cauliflower (Brassica oleracea) (1994), Eur. J. Biochem., 219, 743-750.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.31 5'-fluorosulfonylbenzoyladenosine
-
 Brassica oleracea
2.7.11.31 mammalian protein phosphatase 2C
-
 Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.31 0.013
-
 HMRSAMSGLHGVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.034
-
 HMRSAMTGLHGVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.038
-
 HMRSAMSGLHLGKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.0404
-
 HGRSAMSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.042
-
 HMRSAMSGLHLGKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.049
-
 HMRSAMSGLHGGKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.0498
-
 HMRSAMSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.065
-
 HMRSAMTGLHGVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.069
-
 HMRSAGSGLHLVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.07
-
 HMRSAGSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.091
-
 HMRSAMSGLHLVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.096
-
 HMRSAMSGLHGVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.111
-
 HMKSAMSGLHLVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.118
-
 HMHSAMSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.133
-
 HMKSAMSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 0.428
-
 HMHSAMSGLHLVKRR pH 7.0, 30°C, kinase B Brassica oleracea
2.7.11.31 0.573
-
 HMGSAMSGLHLVKRR pH 7.0, 30°C, kinase A Brassica oleracea
2.7.11.31 2.316
-
 HMGSAMSGLHLVKRR pH 7.0, 30°C, kinase B Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.31 45000
-
 major form B, gel filtration Brassica oleracea
2.7.11.31 58000
-
 x * 58000, catalytic subunit Brassica oleracea
2.7.11.31 150000
-
 major form A, electrophoresis in non-denaturing gels Brassica oleracea
2.7.11.31 160000
-
 major form A, PAGE Brassica oleracea
2.7.11.31 200000
-
 major form A, gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Brassica oleracea
-
 (R)-mevalonate + CoA + NADP+
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Brassica oleracea inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Brassica oleracea
-
 cauliflower
-
2.7.11.31 Brassica oleracea
-
 cauliflower, var. botrytis
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.34 phosphoprotein activity is regulated by phosphorylation and dephosphorylation Brassica oleracea

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.31 2 isoformes, major form A and major form B Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 inflorescence
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.31 3.281
-
 major form B Brassica oleracea
2.7.11.31 13.1
-
 major form A Brassica oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.11.31 -70°C, 0.5 mg protein/ml, frozen in liquid nitrogen, can be stored with no loss of activity Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Brassica oleracea (R)-mevalonate + CoA + NADP+
-
 ?
2.7.11.31 ATP + HGRSAMSGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMGSAMSGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMHSAMSGLHLVKRR
-
 Brassica oleracea ?
-
 ?
2.7.11.31 ATP + HMKSAMSGLHLVKRR synthetic SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAGSGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMSGLHGGKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMSGLHGVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMSGLHLGKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMSGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMTGLHGVKRR
-
 Brassica oleracea ?
-
 ?
2.7.11.31 ATP + HMRSAMTGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + HMRSAMYGLHLVKRR SAMS-containing peptide as substrate Brassica oleracea ADP + ?
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] 2 isoforms, major form A and minor form B, both phosphorylates mammalian HMG-CoA reductase Brassica oleracea ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Brassica oleracea ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.31 ? x * 58000, catalytic subunit Brassica oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
 Brassica oleracea