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Literature summary extracted from

  • Galvez, S.; Bismuth, E.; Sarda, C.; Gadal, P.
    Purification and characterization of chloroplastic NADP-isocitrate dehydrogenase from mixotrophic tobacco cells (1994), Plant Physiol., 105, 593-600.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.007
-
 NADP+ Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
1.1.1.42 0.0075
-
 NADP+ Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
1.1.1.42 0.012
-
 Mg2+ Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
1.1.1.42 0.013
-
 Mg2+ Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
1.1.1.42 0.028
-
 D-isocitrate Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
1.1.1.42 0.028
-
 D-isocitrate chloroplastic enzyme, at pH 7.5 and 30°C Nicotiana tabacum
1.1.1.42 0.041
-
 D-isocitrate Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
1.1.1.42 0.041
-
 D-isocitrate cytosolic enzyme, at pH 7.5 and 30°C Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 chloroplast ICDH2 Nicotiana tabacum 9507
-
1.1.1.42 cytosol ICDH1 Nicotiana tabacum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 117000
-
 gel filtration, ICDH1 Nicotiana tabacum
1.1.1.42 136000
-
 gel filtration, ICDH2 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Nicotiana tabacum
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 18.6
-
 ICDH2 Nicotiana tabacum
1.1.1.42 50
-
 ICDH1 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 D-isocitrate + NADP+
-
 Nicotiana tabacum 2-oxoglutarate + CO2 + NADPH
-
 r
1.1.1.42 isocitrate + NADP+
-
 Nicotiana tabacum 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Nicotiana tabacum