BRENDA - Enzyme Database

Triphosphopyridine nucleotide-allyl and -ethyl alcohol dehydrogenases from Escherichia coli (preliminary report)

Otsuka, K.; J. Gen. Appl. Microbiol. 4, 211-215 (1958)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.54
cyanide
-
Escherichia coli
1.1.1.54
dipyridyl
-
Escherichia coli
1.1.1.54
EDTA
-
Escherichia coli
1.1.1.54
additional information
not: monoiodoacetate
Escherichia coli
1.1.1.54
p-chloromercuribenzoate
can be overcome by addition of gluthathione
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.54
0.04
-
NADP+
-
Escherichia coli
1.1.1.54
5
-
allyl-alcohol
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.54
additional information
is sensitive against metal ion chelators
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.54
allyl alcohol + NADP+
Escherichia coli
-
acrolein + NADPH
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.54
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.54
partial
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.54
additional information
-
about 70,1 unit of enzyme activity defined as amount of enzyme which causes a change in optical density of 0.001 per minute
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.54
2,3-butylene glycol + NADP+
-
287112
Escherichia coli
?
-
-
-
r
1.1.1.54
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
-
-
r
1.1.1.54
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
287112
Escherichia coli
r
1.1.1.54
saturated primary alcohols + NADP+
i.e. ethyl alcohol, n-propyl alcohol, n-butyl acohol, isobutyl alcohol, propylene glycol
287112
Escherichia coli
?
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.54
7.7
-
in Tris-buffer
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.54
NAD(P)H
-
Escherichia coli
1.1.1.54
NADP+
specific for
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.54
NAD(P)H
-
Escherichia coli
1.1.1.54
NADP+
specific for
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.54
cyanide
-
Escherichia coli
1.1.1.54
dipyridyl
-
Escherichia coli
1.1.1.54
EDTA
-
Escherichia coli
1.1.1.54
additional information
not: monoiodoacetate
Escherichia coli
1.1.1.54
p-chloromercuribenzoate
can be overcome by addition of gluthathione
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.54
0.04
-
NADP+
-
Escherichia coli
1.1.1.54
5
-
allyl-alcohol
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.54
additional information
is sensitive against metal ion chelators
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.54
allyl alcohol + NADP+
Escherichia coli
-
acrolein + NADPH
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.54
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.54
additional information
-
about 70,1 unit of enzyme activity defined as amount of enzyme which causes a change in optical density of 0.001 per minute
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.54
2,3-butylene glycol + NADP+
-
287112
Escherichia coli
?
-
-
-
r
1.1.1.54
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
-
-
r
1.1.1.54
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
287112
Escherichia coli
r
1.1.1.54
saturated primary alcohols + NADP+
i.e. ethyl alcohol, n-propyl alcohol, n-butyl acohol, isobutyl alcohol, propylene glycol
287112
Escherichia coli
?
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.54
7.7
-
in Tris-buffer
Escherichia coli