EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.54 | 3-methyl-2-buten-1-al | product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay | Pseudomonas putida | |
1.1.1.54 | dithiothreitol | - |
Pseudomonas putida | |
1.1.1.54 | EDTA | only at concentration of 100 mM 50% reduction | Pseudomonas putida | |
1.1.1.54 | N-ethylmaleimide | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.54 | 0.007 | - |
benzyl alcohol | - |
Pseudomonas putida | |
1.1.1.54 | 0.007 | - |
3-chloro-2-buten-1-ol | - |
Pseudomonas putida | |
1.1.1.54 | 0.011 | - |
3-aminobenzyl alcohol | - |
Pseudomonas putida | |
1.1.1.54 | 0.012 | - |
(R)-(+)-perillyl alcohol | - |
Pseudomonas putida | |
1.1.1.54 | 0.036 | - |
cinnamyl alcohol | - |
Pseudomonas putida | |
1.1.1.54 | 0.04 | - |
3-methyl-2-buten-1-ol | - |
Pseudomonas putida | |
1.1.1.54 | 0.044 | - |
2-Buten-1-ol | - |
Pseudomonas putida | |
1.1.1.54 | 0.064 | - |
NAD+ | with 3-methyl-2-buten-1-ol | Pseudomonas putida | |
1.1.1.54 | 0.118 | - |
NAD+ | with benzyl alcohol | Pseudomonas putida | |
1.1.1.54 | 0.27 | - |
3-methyl-1-butanol | - |
Pseudomonas putida | |
1.1.1.54 | 1.1 | - |
1-butanol | - |
Pseudomonas putida | |
1.1.1.54 | 1.7 | - |
3-methyl-3-buten-1-ol | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.54 | additional information | is not sensitive against metal ion chelators | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 37700 | - |
4 * 37700, SDS-PAGE | Pseudomonas putida |
1.1.1.54 | 151000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | Pseudomonas putida | can use substrate as sole carbon source | 3-methyl-2-buten-1-al + NADH | - |
? | |
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | Pseudomonas putida MB-1 | can use substrate as sole carbon source | 3-methyl-2-buten-1-al + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.54 | Pseudomonas putida | - |
- |
- |
1.1.1.54 | Pseudomonas putida MB-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.54 | - |
Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.54 | allyl alcohol + NADP+ = acrolein + NADPH + H+ | 3-methyl-2-buten-1-ol-dependent inducible activity | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 0.05 | - |
purified enzyme, substrate 1-butanol | Pseudomonas putida |
1.1.1.54 | 0.08 | - |
purified enzyme, substrate 3-methyl-3-buten-1-ol | Pseudomonas putida |
1.1.1.54 | 0.2 | - |
purified enzyme, substrate 3-methyl-1-butanol | Pseudomonas putida |
1.1.1.54 | 3.7 | - |
purified enzyme, substrate cinnamyl alcohol | Pseudomonas putida |
1.1.1.54 | 4.6 | - |
purified enzyme, substrate 2-buten-1-ol | Pseudomonas putida |
1.1.1.54 | 7.7 | - |
purified enzyme, substrate 3-methyl-2-buten-1-ol | Pseudomonas putida |
1.1.1.54 | 8.8 | - |
purified enzyme, substrate (R)-(+)-perillyl alcohol | Pseudomonas putida |
1.1.1.54 | 16 | - |
purified enzyme, substrate 3-chlor-2-buten-1-ol | Pseudomonas putida |
1.1.1.54 | 19.3 | - |
purified enzyme, in glycine buffer pH 9.4 | Pseudomonas putida |
1.1.1.54 | 22 | - |
purified enzyme, substrate benzyl alcohol | Pseudomonas putida |
1.1.1.54 | 38 | - |
purified enzyme, substrate aromatic 3-aminobenzyl alcohol | Pseudomonas putida |
1.1.1.54 | 38.8 | - |
purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4 | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.54 | -70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.54 | (R)-(+)-perillyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | 1-butanol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | 2-buten-1-ol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | 2-buten-2-ol + NADPH | - |
Pseudomonas putida | but-2-enal | - |
? | |
1.1.1.54 | 2-buten-2-ol + NADPH | - |
Pseudomonas putida MB-1 | but-2-enal | - |
? | |
1.1.1.54 | 3-aminobenzyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | 3-chloro-2-buten-1-ol + NADP+ | - |
Pseudomonas putida | 3-chloro-2-butenaldehyde + NADPH | - |
? | |
1.1.1.54 | 3-chloro-2-buten-1-ol + NADP+ | - |
Pseudomonas putida MB-1 | 3-chloro-2-butenaldehyde + NADPH | - |
? | |
1.1.1.54 | 3-methyl-1-butanol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | - |
Pseudomonas putida | 3-methyl-2-buten-1-al + NADH | - |
? | |
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | can use substrate as sole carbon source | Pseudomonas putida | 3-methyl-2-buten-1-al + NADH | - |
? | |
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | - |
Pseudomonas putida MB-1 | 3-methyl-2-buten-1-al + NADH | - |
? | |
1.1.1.54 | 3-methyl-2-buten-1-ol + NAD+ | can use substrate as sole carbon source | Pseudomonas putida MB-1 | 3-methyl-2-buten-1-al + NADH | - |
? | |
1.1.1.54 | 3-methyl-3-buten-1-ol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | benzyl alcohol + NADP+ | - |
Pseudomonas putida | ? | - |
r | |
1.1.1.54 | cinnamyl alcohol + NADP+ | - |
Pseudomonas putida | cinnamyl aldehyde + NADPH | - |
? | |
1.1.1.54 | cinnamyl alcohol + NADP+ | - |
Pseudomonas putida MB-1 | cinnamyl aldehyde + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.54 | tetramer | 4 * 37700, SDS-PAGE | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 5.4 | - |
for reduction | Pseudomonas putida |
1.1.1.54 | 10 | - |
for oxidation | Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 9.4 | - |
stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0 | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.54 | NAD+ | exhibits less than 5% of the activity with NADP+ | Pseudomonas putida |