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Literature summary extracted from

  • Malone, V.F.; Chastian, A.J.; Ohlsson, J.T.; Poneleit, L.S.; Nemecek-Marshall, M.; Fall, R.
    Characterization of a Pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol (1999), Appl. Environ. Microbiol., 65, 2622-2630.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.54 3-methyl-2-buten-1-al product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay Pseudomonas putida
1.1.1.54 dithiothreitol
-
Pseudomonas putida
1.1.1.54 EDTA only at concentration of 100 mM 50% reduction Pseudomonas putida
1.1.1.54 N-ethylmaleimide
-
Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.54 0.007
-
benzyl alcohol
-
Pseudomonas putida
1.1.1.54 0.007
-
3-chloro-2-buten-1-ol
-
Pseudomonas putida
1.1.1.54 0.011
-
3-aminobenzyl alcohol
-
Pseudomonas putida
1.1.1.54 0.012
-
(R)-(+)-perillyl alcohol
-
Pseudomonas putida
1.1.1.54 0.036
-
cinnamyl alcohol
-
Pseudomonas putida
1.1.1.54 0.04
-
3-methyl-2-buten-1-ol
-
Pseudomonas putida
1.1.1.54 0.044
-
2-Buten-1-ol
-
Pseudomonas putida
1.1.1.54 0.064
-
NAD+ with 3-methyl-2-buten-1-ol Pseudomonas putida
1.1.1.54 0.118
-
NAD+ with benzyl alcohol Pseudomonas putida
1.1.1.54 0.27
-
3-methyl-1-butanol
-
Pseudomonas putida
1.1.1.54 1.1
-
1-butanol
-
Pseudomonas putida
1.1.1.54 1.7
-
3-methyl-3-buten-1-ol
-
Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.54 additional information is not sensitive against metal ion chelators Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.54 37700
-
4 * 37700, SDS-PAGE Pseudomonas putida
1.1.1.54 151000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.54 3-methyl-2-buten-1-ol + NAD+ Pseudomonas putida can use substrate as sole carbon source 3-methyl-2-buten-1-al + NADH
-
?
1.1.1.54 3-methyl-2-buten-1-ol + NAD+ Pseudomonas putida MB-1 can use substrate as sole carbon source 3-methyl-2-buten-1-al + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.54 Pseudomonas putida
-
-
-
1.1.1.54 Pseudomonas putida MB-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.54
-
Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.54 allyl alcohol + NADP+ = acrolein + NADPH + H+ 3-methyl-2-buten-1-ol-dependent inducible activity Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.54 0.05
-
purified enzyme, substrate 1-butanol Pseudomonas putida
1.1.1.54 0.08
-
purified enzyme, substrate 3-methyl-3-buten-1-ol Pseudomonas putida
1.1.1.54 0.2
-
purified enzyme, substrate 3-methyl-1-butanol Pseudomonas putida
1.1.1.54 3.7
-
purified enzyme, substrate cinnamyl alcohol Pseudomonas putida
1.1.1.54 4.6
-
purified enzyme, substrate 2-buten-1-ol Pseudomonas putida
1.1.1.54 7.7
-
purified enzyme, substrate 3-methyl-2-buten-1-ol Pseudomonas putida
1.1.1.54 8.8
-
purified enzyme, substrate (R)-(+)-perillyl alcohol Pseudomonas putida
1.1.1.54 16
-
purified enzyme, substrate 3-chlor-2-buten-1-ol Pseudomonas putida
1.1.1.54 19.3
-
purified enzyme, in glycine buffer pH 9.4 Pseudomonas putida
1.1.1.54 22
-
purified enzyme, substrate benzyl alcohol Pseudomonas putida
1.1.1.54 38
-
purified enzyme, substrate aromatic 3-aminobenzyl alcohol Pseudomonas putida
1.1.1.54 38.8
-
purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.54 -70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.54 (R)-(+)-perillyl alcohol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 1-butanol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 2-buten-1-ol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 2-buten-2-ol + NADPH
-
Pseudomonas putida but-2-enal
-
?
1.1.1.54 2-buten-2-ol + NADPH
-
Pseudomonas putida MB-1 but-2-enal
-
?
1.1.1.54 3-aminobenzyl alcohol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 3-chloro-2-buten-1-ol + NADP+
-
Pseudomonas putida 3-chloro-2-butenaldehyde + NADPH
-
?
1.1.1.54 3-chloro-2-buten-1-ol + NADP+
-
Pseudomonas putida MB-1 3-chloro-2-butenaldehyde + NADPH
-
?
1.1.1.54 3-methyl-1-butanol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 3-methyl-2-buten-1-ol + NAD+
-
Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
?
1.1.1.54 3-methyl-2-buten-1-ol + NAD+ can use substrate as sole carbon source Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
?
1.1.1.54 3-methyl-2-buten-1-ol + NAD+
-
Pseudomonas putida MB-1 3-methyl-2-buten-1-al + NADH
-
?
1.1.1.54 3-methyl-2-buten-1-ol + NAD+ can use substrate as sole carbon source Pseudomonas putida MB-1 3-methyl-2-buten-1-al + NADH
-
?
1.1.1.54 3-methyl-3-buten-1-ol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 benzyl alcohol + NADP+
-
Pseudomonas putida ?
-
r
1.1.1.54 cinnamyl alcohol + NADP+
-
Pseudomonas putida cinnamyl aldehyde + NADPH
-
?
1.1.1.54 cinnamyl alcohol + NADP+
-
Pseudomonas putida MB-1 cinnamyl aldehyde + NADPH
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.54 tetramer 4 * 37700, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.54 5.4
-
for reduction Pseudomonas putida
1.1.1.54 10
-
for oxidation Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.54 9.4
-
stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.54 NAD+ exhibits less than 5% of the activity with NADP+ Pseudomonas putida