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Literature summary extracted from

  • Nirenberg, M.W.; Jakoby, W.B.
    Enzymatic utilization of gamma-hydroxybutyric acid (1960), J. Biol. Chem., 235, 954-960.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.61 p-chloromercuribenzoate 10 mM leads to 50% inhibition Pseudomonas sp.
1.2.1.16 potassium arsenite 75% inhibition, concentration 2 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NAD+ Pseudomonas sp.
-
 succinate semialdehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Pseudomonas sp.
-
-
-
1.2.1.16 Pseudomonas sp.
-
 constitutive
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.61
-
 Pseudomonas sp.
1.2.1.16 DEAE-cellulose chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 additional information
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.2.1.16 3°C or -15°C, overnight, 100% loss of activity, without mercaptoethanol Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NAD+
-
 Pseudomonas sp. succinate semialdehyde + NADH + H+
-
 r
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Pseudomonas sp. succinate + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.61 7
-
 reduction of 4-hydroxybutanoate Pseudomonas sp.
1.1.1.61 10.5
-
 oxidation of 4-hydroxybutanoate Pseudomonas sp.
1.2.1.16 9 9.5
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.61 NAD+
-
 Pseudomonas sp.
1.1.1.61 NADH
-
 Pseudomonas sp.
1.2.1.16 NAD+
-
 Pseudomonas sp.
1.2.1.16 NADP+ reduces NAD+ at one-twelfth the rate of NADP+ Pseudomonas sp.