EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | Fe2+ | - |
Pseudomonas sp. | |
1.1.3.6 | Hg2+ | - |
Pseudomonas sp. | |
1.1.3.6 | Zn2+ | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.2 | - |
cholesterol | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | Mn2+ | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 56000 | - |
SDS-PAGE, gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | Pseudomonas sp. | - |
cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | Pseudomonas sp. | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 27.9 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | 5alpha-cholestan-3beta-ol + O2 | - |
Pseudomonas sp. | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | enzyme specifically oxidizes 3beta-hydroxy groups in steroids | Pseudomonas sp. | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
Pseudomonas sp. | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | dihydrocholesterol + O2 | - |
Pseudomonas sp. | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | ergosterol + O2 | - |
Pseudomonas sp. | dehydroergosterol + H2O2 | - |
? | |
1.1.3.6 | stigmastanol + O2 | - |
Pseudomonas sp. | stigmastanone + H2O2 | - |
? | |
1.1.3.6 | stigmasterol + O2 | - |
Pseudomonas sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.6 | monomer | 1 * 56000, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 70 | - |
5 min, no loss of activity | Pseudomonas sp. |
1.1.3.6 | 70 | - |
30 min, 15% loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 7 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 5 | 9 | 33% of maximal activity at pH 5.0 and pH 9.0 | Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 5 | 8 | 60°C, 1 min, stable | Pseudomonas sp. |
1.1.3.6 | 10 | - |
60°C, 1 min, 25% loss of activity | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | FAD | exogenous addition increases activity | Pseudomonas sp. |