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Literature summary extracted from

  • Doukyu, N.; Aono, R.
    Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200 (1998), Appl. Environ. Microbiol., 64, 1929-1932.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.6 Benzene strain ST-200, 3fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 Pseudomonas sp.
1.1.3.6 diphenylmethane strain ST-200, 3.1fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 Pseudomonas sp.
1.1.3.6 p-xylene strain ST-200, 3.4fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 Pseudomonas sp.
1.1.3.6 propylbenzene strain ST-200, 3.2fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 Pseudomonas sp.
1.1.3.6 Toluene strain ST-200, 3.5fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.004
-
 cholesterol strain ST-200 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Pseudomonas sp.
-
 cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 Pseudomonas sp. ST-200
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pseudomonas sp.
-
 ST-200
-
1.1.3.6 Pseudomonas sp. ST-200
-
 ST-200
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6 strain ST-200, ammonium sulfate, DEAE-cellulose, butyl-toyopearl, Sephadex G-100 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 15.2
-
 strain ST-200 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. ST-200 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. ST-200 cholest-5-en-3-one + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 60
-
 strain ST-200 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 4 50 strain ST-200, 27% activity lost after incubation at 60°C, complete loss of activity at 70°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 6.8 8 strain ST-200 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 8.5 strain ST-200 Pseudomonas sp.