EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.3 | FAD | stimulates the reaction with phenazine methosulfate and methylene blue. 20% increase in activity of the reaction with phenazine methosulfate with a half-maximal concentration of 200 nM | Escherichia coli | |
1.1.5.3 | FMN | stimulates the reaction with phenazine methosulfate. 6fold increase in activity with a half-maximal concentration of 0.13 mM | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.3 | 1,10-phenanthroline | - |
Escherichia coli | |
1.1.5.3 | DTNB | - |
Escherichia coli | |
1.1.5.3 | FMN | reaction with methylene blue | Escherichia coli | |
1.1.5.3 | PCMB | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.3 | 0.1 | - |
glycerol 3-phosphate | in absence of exogenous FMN | Escherichia coli | |
1.1.5.3 | 0.339 | - |
glycerol 3-phosphate | in presence of exogenous FMN | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.3 | membrane | associated with membrane | Escherichia coli | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.3 | Iron | contains 2 mol of non-heme iron per dimer | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.5.3 | 43000 | - |
1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
1.1.5.3 | 62000 | - |
1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
1.1.5.3 | 93500 | - |
equilibrium sedimentation | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.3 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.3 | - |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.5.3 | -70°C or 4°C, concentrated to 4-10 mg/ml, stable | Escherichia coli |
1.1.5.3 | 4°C, pH 6.0-8.5, 20% ethylene glycol v/v, 10% loss of activity after 60 h | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.3 | sn-glycerol 3-phosphate + methylene blue | - |
Escherichia coli | glycerone phosphate + reduced methylene blue | - |
? | |
1.1.5.3 | sn-glycerol 3-phosphate + phenazine methosulfate | - |
Escherichia coli | glycerone phosphate + reduced phenazine methosulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.3 | dimer | 1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.3 | 33.3 | - |
glycerol 3-phosphate | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.3 | FAD | contains 1 mol of noncovalently bound FAD per dimer | Escherichia coli |