Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Storts, D.R.; Bhattacharjee, J.K.
    Properties of revertants of lys2 and lys5 mutants as well as alpha-aminoadipate-semialdehyde dehydrogenase from Saccharomyces cerevisiae (1989), Biochem. Biophys. Res. Commun., 161, 182-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.31 encoded by two different genes, LYS2 and LYS5 Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
1.2.1.31 addition of 10 mM 2-mercaptoethanol and protease inhibitors e.g.: 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride to the crude extract improves stability Saccharomyces cerevisiae
1.2.1.31 low ionic strength: unstable Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.31 Mg2+ required for activity Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.31 180000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Saccharomyces cerevisiae in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Saccharomyces cerevisiae sixth step of lysine biosynthesis L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.31 partial, using column chromatography on DEAE-cellulose, gel filtration, hydrolxylapatite and Reactive Red-120 agarose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.31 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Saccharomyces cerevisiae L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Saccharomyces cerevisiae L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H sixth step of lysine biosynthesis Saccharomyces cerevisiae L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.31 -70
-
 unstable Saccharomyces cerevisiae
1.2.1.31 -20
-
 unstable Saccharomyces cerevisiae
1.2.1.31 4
-
 unstable Saccharomyces cerevisiae
1.2.1.31 30
-
 unstable Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.31 ATP
-
 Saccharomyces cerevisiae
1.2.1.31 NADPH
-
 Saccharomyces cerevisiae