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Literature summary extracted from

  • Schmidt, H.; Bode, R.; Lindner, M.; Birnbaum, D.
    Lysine biosynthesis in the yeast Candida maltosa: properties of some enzymes and regulation of the biosynthetic pathway (1985), J. Basic Microbiol., 25, 675-681.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.31 Cu2+
-
Candida maltosa
1.2.1.31 Fe2+
-
Candida maltosa
1.2.1.31 glutamate competitive inhibitor with respect to alpha-aminoadipate Candida maltosa
1.2.1.31 Hg2+
-
Candida maltosa
1.2.1.31 L-aspartate competitive inhibitor with respect to alpha-aminoadipate Candida maltosa
1.2.1.31 Sn2+
-
Candida maltosa
1.5.1.7 amino acids hydrophobic amino acids with 5 or 6 carbon atoms; hydroxylic amino acids Candida maltosa
1.5.1.7 L-isoleucine
-
Candida maltosa
1.5.1.7 L-leucine
-
Candida maltosa
1.5.1.7 L-norleucine
-
Candida maltosa
1.5.1.7 L-ornithine
-
Candida maltosa
2.3.3.14 Co2+
-
Candida maltosa
2.3.3.14 DL-alpha-aminoadipate
-
Candida maltosa
2.3.3.14 Hg2+
-
Candida maltosa
2.3.3.14 L-Lys
-
Candida maltosa
2.3.3.14 Mn2+
-
Candida maltosa
2.3.3.14 thialysine L-thialysine Candida maltosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.31 0.1
-
NADPH
-
Candida maltosa
1.2.1.31 0.2
-
alpha-aminoadipate
-
Candida maltosa
1.2.1.31 0.38
-
NADH
-
Candida maltosa
1.2.1.31 0.5
-
ATP
-
Candida maltosa
1.2.1.31 3.8
-
MgCl2
-
Candida maltosa
1.5.1.7 0.23
-
NADH
-
Candida maltosa
1.5.1.7 0.66
-
2-oxoglutarate
-
Candida maltosa
1.5.1.7 1.6
-
L-lysine
-
Candida maltosa
2.3.3.14 0.025
-
2-oxoglutarate
-
Candida maltosa
2.3.3.14 0.033
-
acetyl-CoA
-
Candida maltosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.31 Co2+ stimulates Candida maltosa
1.2.1.31 Cu2+ inhibits Candida maltosa
1.2.1.31 Mg2+ stimulates Candida maltosa
1.2.1.31 Mg2+ required for activity Candida maltosa
1.2.1.31 Mn2+ stimulates Candida maltosa
1.5.1.7 Cu2+ 4% of control activity at 1 mM Candida maltosa
1.5.1.7 Hg2+ complete inhibition at 1 mM Candida maltosa
1.5.1.7 Mn2+ 46% of control activity at 1 mM Candida maltosa
1.5.1.7 Sn2+ 11% of control activity at 1 mM Candida maltosa
1.5.1.7 Zn2+ 66% of control activity at 1 mM Candida maltosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.31 160000
-
gel filtration Candida maltosa
1.5.1.7 45000
-
gel filtration Candida maltosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Candida maltosa in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Candida maltosa involved in lysine biosynthetic pathway L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Candida maltosa L4 in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Candida maltosa L4 involved in lysine biosynthetic pathway L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Candida maltosa involved in lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Candida maltosa last step of alpha-aminoadipate pathway for lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
?
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate Candida maltosa
-
2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Candida maltosa
-
-
-
1.5.1.7 Candida maltosa
-
-
-
1.5.1.7 Candida maltosa L4
-
-
-
2.3.3.14 Candida maltosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.31 using column chromatography on Sephadex G-25 and Sephadex G-200 Candida maltosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate = (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA sequential mechanism Candida maltosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.31 0.0063
-
5 mM L-lysine as supplement during growth of the enzyme Candida maltosa
1.2.1.31 0.0072
-
no supplement added during growth of the enzyme Candida maltosa
1.2.1.31 0.0138
-
10 mM amitrole as supplement during growth of the enzyme Candida maltosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 alpha-aminoadipic semialdehyde + NAD+ + H2O
-
Candida maltosa alpha-aminoadipic acid + NADH + H+
-
?
1.2.1.31 alpha-aminoadipic semialdehyde + NAD+ + H2O
-
Candida maltosa L4 alpha-aminoadipic acid + NADH + H+
-
?
1.2.1.31 L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Candida maltosa L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
?
1.2.1.31 L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Candida maltosa L4 L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Candida maltosa L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H involved in lysine biosynthetic pathway Candida maltosa L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Candida maltosa L4 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.2.1.31 L-alpha-aminoadipate + NAD(P)H involved in lysine biosynthetic pathway Candida maltosa L4 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O i.e. saccharopine Candida maltosa L-lysine + 2-oxoglutarate + NADH
-
r
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine biosynthesis Candida maltosa L-lysine + 2-oxoglutarate + NADH
-
?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O last step of alpha-aminoadipate pathway for lysine biosynthesis Candida maltosa L-lysine + 2-oxoglutarate + NADH
-
?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O i.e. saccharopine Candida maltosa L4 L-lysine + 2-oxoglutarate + NADH
-
r
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
Candida maltosa 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.31 6.5 7.5
-
Candida maltosa
1.5.1.7 7
-
L-lysine + 2-oxoglutarate + NADH Candida maltosa
2.3.3.14 8 8.7
-
Candida maltosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.31 NADH can replace NADPH with lower activity and higher Km Candida maltosa
1.2.1.31 NADPH
-
Candida maltosa
1.5.1.7 NAD+
-
Candida maltosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.31 1.5
-
L-glutamate
-
Candida maltosa
1.2.1.31 4.2
-
L-aspartate
-
Candida maltosa
2.3.3.14 5
-
L-lysine
-
Candida maltosa
2.3.3.14 5.1
-
DL-alpha-aminoadipate
-
Candida maltosa
2.3.3.14 15
-
L-thialysine
-
Candida maltosa