Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pundir, C.S.; Goyal, L.; Thakur, M.; Bhargava, A.K.
    Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass (1999), Indian J. Biochem. Biophys., 36, 449-452.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 glucose physiological concentration, 20% activation free protein, 25% activation immobilized protein Amaranthus spinosus
1.2.3.4 glycine 1 mM concentration, 10% activation free protein, 18% activation immobilized protein Amaranthus spinosus
1.2.3.4 hippuric acid 1 mM concentration, 10% activation free protein, 8% activation immobilized protein Amaranthus spinosus
1.2.3.4 serine 1 mM concentration, 12% activation free protein, 10% activation immobilized protein Amaranthus spinosus
1.2.3.4 Urea physiological concentration, 8% activation free protein Amaranthus spinosus
1.2.3.4 uric acid 1 mM concentration, 12% activation free protein, 8% activation immobilized protein Amaranthus spinosus

Application

EC Number Application Comment Organism
1.2.3.4 medicine immobilized oxalate oxidase to determine oxalate concentration in urine for diagnosis of various forms of hyperoxaluria Amaranthus spinosus

General Stability

EC Number General Stability Organism
1.2.3.4 immobilized on alkylamine glass Amaranthus spinosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 ascorbate wild type and immobilized 88% and 81% inhibition respectively Amaranthus spinosus
1.2.3.4 Dithionite
-
 Amaranthus spinosus
1.2.3.4 glutathione slight inhibition, wild type and immobilized Amaranthus spinosus
1.2.3.4 L-cysteine wild type and immobilized slight inhibition Amaranthus spinosus
1.2.3.4 NaCl 10% inhibition at 1 mM concentration Amaranthus spinosus
1.2.3.4 nitrate mM concentration Amaranthus spinosus
1.2.3.4 nitrite 1 mM concentration 10% inhibition Amaranthus spinosus
1.2.3.4 pyruvate 1 mM concentration 15% inhibition of the free enzyme, not of the immobilized enzyme Amaranthus spinosus
1.2.3.4 salicylate
-
 Amaranthus spinosus
1.2.3.4 ZnSO4 1 mM concentration, 10% inhibition of the free enzyme, not of the immobilized enzyme Amaranthus spinosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.21
-
 oxalate
-
 Amaranthus spinosus
1.2.3.4 0.39
-
 oxalate immobilized protein Amaranthus spinosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.3.4 membrane
-
 Amaranthus spinosus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Amaranthus spinosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4
-
 Amaranthus spinosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 leaf
-
 Amaranthus spinosus
-

Storage Stability

EC Number Storage Stability Organism
1.2.3.4 4°C, 3 months, distilled water, free protein, complete loss of activity, immobilized protein, 70% of activity retained Amaranthus spinosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Amaranthus spinosus 2 CO2 + H2O2
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.4 35
-
 immobilized protein Amaranthus spinosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 75
-
 after 30 min wild type 60% activity, immobilized protein 27% activity Amaranthus spinosus