Any feedback?
Literature summary extracted from
- Assay and properties of acetone carboxylase, a novel enzyme involved in acetone-dependent growth and CO2 fixation in Rhodobacter capsulatus and other photosynthetic and denitrifying bacteria (1997), Microbiology, 143, 755-766.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.6 | acetyl-CoA | - |
Paracoccus pantotrophus |  |
| 6.4.1.6 | acetyl-CoA | dependent on, gives more reproducible and larger degree of stimulation than CoA | Rhodobacter capsulatus | |
| 6.4.1.6 | CoA | stimulates acetone-dependent CO2 fixation | Rhodobacter capsulatus | |
| 6.4.1.6 | NAD(P)H | - |
Rhodobacter capsulatus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.6 | ATP | in high concentrations | Rhodobacter capsulatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.4.1.6 | 0.03 | - |
acetone | - |
Rhodobacter capsulatus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.6 | Mg2+ | required for assay, stimulating | Rhodobacter capsulatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | 70000 | - |
x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
| 6.4.1.6 | 85000 | - |
x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.6 | acetone + CO2 + ATP + H2O | Rhodobacter capsulatus | initial step in acetone metabolism | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | Rhodobacter capsulatus PAS100 | initial step in acetone metabolism | acetoacetate + AMP + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.4.1.6 | Paracoccus pantotrophus | - |
LMD82.1 | - |
| 6.4.1.6 | Paracoccus pantotrophus LMD82.1 | - |
LMD82.1 | - |
| 6.4.1.6 | Rhodobacter capsulatus | - |
PAS100 | - |
| 6.4.1.6 | Rhodobacter capsulatus PAS100 | - |
PAS100 | - |
| 6.4.1.6 | Rhodomicrobium vannielii | - |
RB4 | - |
| 6.4.1.6 | Rhodomicrobium vannielii RB4 | - |
RB4 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.4.1.6 | ion-exchange chromatography | Rhodobacter capsulatus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 6.4.1.6 | acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate | biotin-independent, activity is strongly induced by growth on acetone | Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | additional information | - |
a few hundred pmol per minute and mg protein | Rhodobacter capsulatus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 6.4.1.6 | -20°C | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Rhodomicrobium vannielii | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Paracoccus pantotrophus | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | phosphate buffer, CoA and magnesium ions needed | Rhodobacter capsulatus | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | initial step in acetone metabolism | Rhodobacter capsulatus | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | phosphate buffer, CoA and magnesium ions needed | Rhodobacter capsulatus PAS100 | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | initial step in acetone metabolism | Rhodobacter capsulatus PAS100 | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Rhodomicrobium vannielii RB4 | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Paracoccus pantotrophus LMD82.1 | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | butanone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus | 3-oxopentanoate + AMP + phosphate | - |
? | |
| 6.4.1.6 | butanone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus PAS100 | 3-oxopentanoate + AMP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.4.1.6 | ? | x * 70000 + x * 85000, also 60000 Da and small amounts of 35000 Da subunits exist, SDS-PAGE | Rhodobacter capsulatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | 30 | - |
assay at | Rhodobacter capsulatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | 7.2 | - |
- |
Rhodobacter capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.4.1.6 | ATP | - |
Rhodobacter capsulatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
