EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.2 | CoCl2 | - |
Pseudomonas putida | |
5.5.1.2 | CuCl2 | - |
Pseudomonas putida | |
5.5.1.2 | NiCl2 | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.24 | 0.012 | - |
2-(5-oxo-4,5-dihydrofuran-2-yl)acetic acid | - |
Pseudomonas putida | |
5.5.1.2 | 0.075 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.24 | 33000 | - |
- |
Pseudomonas putida |
5.5.1.2 | 190000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.2 | additional information | Pseudomonas putida | enzyme of the protocatechuate pathway | ? | - |
? | |
5.5.1.2 | additional information | Pseudomonas putida A.312 | enzyme of the protocatechuate pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.24 | Pseudomonas putida | - |
A.312 | - |
3.1.1.24 | Pseudomonas putida A.312 | - |
A.312 | - |
5.5.1.2 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.24 | 422fold | Pseudomonas putida |
5.5.1.2 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.24 | 717 | - |
- |
Pseudomonas putida |
5.5.1.2 | additional information | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida | 3-oxoadipate | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida A.312 | 3-oxoadipate | - |
? | |
5.5.1.2 | 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | i.e. beta-carboxy-cis,cis-muconate | Pseudomonas putida | cis-Butadiene-1,2,4-tricarboxylate | i.e. gamma-carboxymuconolactone | ? | |
5.5.1.2 | 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | i.e. beta-carboxy-cis,cis-muconate | Pseudomonas putida A.312 | cis-Butadiene-1,2,4-tricarboxylate | i.e. gamma-carboxymuconolactone | ? | |
5.5.1.2 | additional information | enzyme of the protocatechuate pathway | Pseudomonas putida | ? | - |
? | |
5.5.1.2 | additional information | enzyme of the protocatechuate pathway | Pseudomonas putida A.312 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.24 | beta-ketoadipate enol-lactone hydrolase | - |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.24 | 55 | - |
half-life, 3 min in 0.02 M sodium potassium phosphate buffer, pH 6.8, 0.005 mM Mg-EDTA | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.24 | 7.5 | 10 | decrease of the activity is dependent on the buffer, the enzyme is less active in Tris-HCl than in potassium phosphate buffer at pH 7.5 | Pseudomonas putida |
5.5.1.2 | 6 | 7 | - |
Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.5.1.2 | 5.5 | 9 | pH 5.5: about 70% of maximal activity, pH 9.0: about 25% of maximal activity | Pseudomonas putida |