BRENDA - Enzyme Database

Separation and quantitation of 2,3-butanediol isomers ((-), (+), and meso) by a combined use of enzyme and gas chromatography

Ui, S.; Masuda, H.; Muraki, H.; Agric. Biol. Chem. 48, 2837-2838 (1984)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.76
unstable to repeated freezing and thawing during storage
Corynebacterium glutamicum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.76
Corynebacterium glutamicum
-
C-1012
-
1.1.1.76
Corynebacterium glutamicum C-1012
-
C-1012
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.76
protamine sufate precipitation
Corynebacterium glutamicum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.76
2.8
-
-
Corynebacterium glutamicum
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.76
-17°C, pH 8.0, stable
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.76
L-acetoin + NADH
in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol
389574
Corynebacterium glutamicum
L-2,3-butanediol
-
389574
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol
389574
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389574
Corynebacterium glutamicum C-1012
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.76
25
-
assay at
Corynebacterium glutamicum
1.1.1.76
30
-
-
Corynebacterium glutamicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.76
9.8
-
assay at
Corynebacterium glutamicum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
1.1.1.76
NADH
-
Corynebacterium glutamicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
1.1.1.76
NADH
-
Corynebacterium glutamicum
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.76
unstable to repeated freezing and thawing during storage
Corynebacterium glutamicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.76
protamine sufate precipitation
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.76
2.8
-
-
Corynebacterium glutamicum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.76
-17°C, pH 8.0, stable
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.76
L-acetoin + NADH
in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol
389574
Corynebacterium glutamicum
L-2,3-butanediol
-
389574
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol
389574
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389574
Corynebacterium glutamicum C-1012
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.76
25
-
assay at
Corynebacterium glutamicum
1.1.1.76
30
-
-
Corynebacterium glutamicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.76
9.8
-
assay at
Corynebacterium glutamicum