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Literature summary extracted from
- Enoate reductases of Clostridia: cloning, sequencing, and expression (2001), J. Biol. Chem., 276, 5779-5787.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.31 | - |
Clostridium tyrobutyricum |
| 1.3.1.31 | expressed in Escherichia coli growing under anaerobic conditions | Moorella thermoacetica |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.31 | 73000 | - |
x * 73000, SDS-PAGE | Moorella thermoacetica |
| 1.3.1.31 | 600000 | - |
gel filtration | Moorella thermoacetica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.31 | Clostridium tyrobutyricum | O52933 | - |
- |
| 1.3.1.31 | Moorella thermoacetica | O52935 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.31 | recombinant enzyme | Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.31 | 2.4 | - |
- |
Moorella thermoacetica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.31 | (E)-2-methylbutenoate + NADH | - |
Clostridium tyrobutyricum | (R)-2-methylbutanoate + NAD+ | - |
? |  |
| 1.3.1.31 | (E)-2-methylbutenoate + NADH | - |
Moorella thermoacetica | (R)-2-methylbutanoate + NAD+ | - |
? | |
| 1.3.1.31 | butenoate + NADH | - |
Clostridium tyrobutyricum | butanoate + NAD+ | - |
? | |
| 1.3.1.31 | butenoate + NADH | - |
Moorella thermoacetica | butanoate + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.31 | ? | x * 73000, SDS-PAGE | Moorella thermoacetica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.31 | 7.3 | - |
- |
Moorella thermoacetica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.31 | 5 | 9 | active between pH 5.0 and pH 9.0 | Moorella thermoacetica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.31 | NADH | - |
Clostridium tyrobutyricum | |
| 1.3.1.31 | NADH | - |
Moorella thermoacetica | |
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