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Literature summary extracted from

  • Jacobs, J.M.; Jacobs, N.J.
    Oxidation of protoporphyrinogen to protoporphyrin, a step in chlorophyll and haem biosynthesis. Purification and partial characterization of the enzyme from barley organelles (1987), Biochem. J., 244, 219-224.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.4 oleic acid
-
 Hordeum vulgare

General Stability

EC Number General Stability Organism
1.3.3.4 insensitive to trypsin digestion Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.0005
-
 protoporphyrinogen-IX
-
 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 etioplast
-
 Hordeum vulgare 9513
-
1.3.3.4 mitochondrion
-
 Hordeum vulgare 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 36000
-
 SDS-PAGE Hordeum vulgare
1.3.3.4 210000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Hordeum vulgare penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.4 0.012
-
 purified enzyme from etioplasts Hordeum vulgare
1.3.3.4 0.015
-
 purified enzyme from mitochondria Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
1.3.3.4 -20°C, in presence of 20% glycerol, several months, minimal loss of activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 coproporphyrinogen-I + 3 O2 oxidized at less than 11% of the rate of protoporphyrinogen-IX Hordeum vulgare coproporphyrin-I + 3 H2O2
-
 ?
1.3.3.4 mesoporphyrinogen-IX + 3 O2 oxidized at the same rate as protoporphyrinogen-IX Hordeum vulgare mesoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Hordeum vulgare protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Hordeum vulgare protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 uroporphyrinogen-I + 3 O2 oxidized at less than 11% of the rate of protoporphyrinogen-IX Hordeum vulgare uroporphyrin-I + 3 H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.4 55
-
 60 min at 55°C causes 80% loss of activity Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.4 6
-
-
 Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.4 2 8 at pH 2 and pH 10 less than 10% of activity Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.4 additional information
-
 unstable on acidification Hordeum vulgare