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Literature summary extracted from

  • Matsubara, Y.; Indo, Y.; Naito, E.; Ozasa, H.; Glassberg, R.; Vockley, J.; Ikeda, Y.; Kraus, J.; Tanaka, K.
    Molecular cloning and nucleotide sequence of cDNAs encoding the precursors of rat long chain acyl-coenzyme A, short chain acyl-coenzyme A, and isovaleryl-coenzyme A dehydrogenases. Sequence homology of four enzymes of the acyl-CoA dehydrogenase family (1989), J. Biol. Chem., 264, 16321-16331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1
-
 Rattus norvegicus
1.3.8.4 via immunopurification of enzyme mRNA from polyribosomes for preparation of an enriched cDNA library in pBR322 plasmid, and screening with synthetic oligonucleotide probes corresponding to the amino-terminal sequence of purified rat enzyme protein Rattus norvegicus
1.3.8.7
-
 Rattus norvegicus
1.3.8.8 expression in Escherichia coli, strain XL1-blue Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.4 mitochondrion
-
 Rattus norvegicus 5739
-
1.3.8.8 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.1 42188
-
 4 * 42188, mature enzyme, calculated from nucleotide sequence Rattus norvegicus
1.3.8.4 43098
-
 4 * 43098, nucleotide sequence Rattus norvegicus
1.3.8.7 43685
-
 4 * 43685, mature enzyme, calculation from nucleotide sequence Rattus norvegicus
1.3.8.8 44672
-
 x * 44672, subunit, nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + acceptor Rattus norvegicus
-
 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
1.3.8.4 additional information Rattus norvegicus the enzyme catalyzes the third step of the leucine oxidative metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Rattus norvegicus
-
-
-
1.3.8.4 Rattus norvegicus
-
-
-
1.3.8.7 Rattus norvegicus
-
-
-
1.3.8.8 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.1 gluteal muscle northern blot Rattus norvegicus
-
1.3.8.1 heart
-
 Rattus norvegicus
-
1.3.8.1 kidney northern blot Rattus norvegicus
-
1.3.8.1 liver
-
 Rattus norvegicus
-
1.3.8.4 liver
-
 Rattus norvegicus
-
1.3.8.7 liver
-
 Rattus norvegicus
-
1.3.8.8 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor
-
 Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
 ?
1.3.8.4 isovaleryl-CoA + acceptor
-
 Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
1.3.8.4 isovaleryl-CoA + acceptor acceptors: electron-transfer flavoprotein, phenazine methosulfate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
1.3.8.4 additional information the enzyme catalyzes the third step of the leucine oxidative metabolism Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.1 tetramer 4 * 42188, mature enzyme, calculated from nucleotide sequence Rattus norvegicus
1.3.8.4 tetramer 4 * 43098, nucleotide sequence Rattus norvegicus
1.3.8.7 tetramer 4 * 43685, mature enzyme, calculation from nucleotide sequence Rattus norvegicus
1.3.8.7 tetramer values for precursor enzyme and leader peptide Rattus norvegicus
1.3.8.8 ? x * 44672, subunit, nucleotide sequence Rattus norvegicus