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Literature summary extracted from
- Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver (1987), J. Biol. Chem., 262, 7982-7989.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.8.1 | (methylenecyclopropyl)acetyl-CoA | 0.01 mM, 81% inhibition, 0.001 mM, 49% inhibition | Homo sapiens |  |
| 1.3.8.1 | iodoacetamide | 2 mM, 63% inhibition | Homo sapiens | |
| 1.3.8.1 | N-ethylmaleimide | 2 mM, 41% inhibition, 0.2 mM, 23% inhibition | Homo sapiens | |
| 1.3.8.4 | (methylenecyclopropyl)acetyl-CoA | at 0.001 mM 21% activity remains; at 0.01 mM 3% activity remains | Homo sapiens | |
| 1.3.8.4 | iodoacetamide | at 2 mM 19% activity remains | Homo sapiens | |
| 1.3.8.4 | Methylmercury iodide | - |
Homo sapiens | |
| 1.3.8.4 | N-ethylmaleimide | at 2 mM 64% activity remains | Homo sapiens | |
| 1.3.8.4 | p-chloromercuribenzoate | at 0.1 mM 18% activity remains | Homo sapiens | |
| 1.3.8.4 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
| 1.3.8.7 | (methylenecyclopropyl)acetyl-CoA | 0.01 mM, 95% inhibition | Homo sapiens | |
| 1.3.8.7 | (R)-(-)-(methylenecyclopropyl)acetyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | iodoacetamide | 2 mM, 73% inhibition | Homo sapiens | |
| 1.3.8.7 | N-ethylmaleimide | 2 mM, 43% inhibition | Homo sapiens | |
| 1.3.8.7 | p-chloromercuribenzoate | 0.1 mM, 17% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.8.1 | 0.0041 | - |
electron transfer flavoprotein | - |
Homo sapiens | |
| 1.3.8.1 | 0.0129 | - |
butyryl-CoA | - |
Homo sapiens | |
| 1.3.8.1 | 0.0333 | - |
pentanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.1 | 0.0339 | - |
hexanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.1 | 0.179 | - |
phenazine methosulfate | - |
Homo sapiens | |
| 1.3.8.4 | 0.0038 | - |
electron-transfer flavoprotein | - |
Homo sapiens | |
| 1.3.8.4 | 0.014 | - |
isovaleryl-CoA | - |
Homo sapiens | |
| 1.3.8.4 | 0.0167 | - |
valeryl-CoA | - |
Homo sapiens | |
| 1.3.8.4 | 0.0167 | 0.4 | n-valeryl-CoA | - |
Homo sapiens | |
| 1.3.8.4 | 0.02 | - |
hexanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.4 | 0.025 | - |
butyryl-CoA | - |
Homo sapiens | |
| 1.3.8.4 | 0.833 | - |
phenazine methosulfate | - |
Homo sapiens | |
| 1.3.8.7 | 0.0034 | - |
electron transfer flavoprotein | - |
Homo sapiens | |
| 1.3.8.7 | 0.00714 | - |
butyryl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.008 | - |
hexanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.0091 | - |
decanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.01 | - |
tetradecenoyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.0216 | - |
hexanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.023 | - |
palmitoyl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 0.0563 | - |
valeryl-CoA | - |
Homo sapiens | |
| 1.3.8.7 | 1.67 | - |
phenazine methosulfate | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.8.4 | mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.8.1 | 41200 | - |
4 * 41200, SDS-PAGE | Homo sapiens |
| 1.3.8.1 | 168000 | - |
gel filtration | Homo sapiens |
| 1.3.8.4 | 42000 | - |
4 * 42000, SDS-PAGE | Homo sapiens |
| 1.3.8.4 | 172000 | - |
gel filtration | Homo sapiens |
| 1.3.8.7 | 44000 | - |
4 * 44000, SDS-PAGE | Homo sapiens |
| 1.3.8.7 | 178000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.8.4 | isovaleryl-CoA + acceptor | Homo sapiens | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.8.1 | Homo sapiens | - |
- |
- |
| 1.3.8.4 | Homo sapiens | - |
- |
- |
| 1.3.8.7 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.8.1 | - |
Homo sapiens |
| 1.3.8.4 | using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography | Homo sapiens |
| 1.3.8.7 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.8.1 | liver | - |
Homo sapiens | - |
| 1.3.8.4 | liver | - |
Homo sapiens | - |
| 1.3.8.7 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.8.1 | 7.4 | - |
- |
Homo sapiens |
| 1.3.8.4 | 0.01 | - |
propionyl-CoA as substrate and phenazine methosulfate as acceptor or hexanoyl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
| 1.3.8.4 | 0.03 | - |
butyryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
| 1.3.8.4 | 0.09 | - |
valeryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
| 1.3.8.4 | 0.17 | - |
S-2-methylbutyryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
| 1.3.8.4 | 0.51 | - |
butyryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
| 1.3.8.4 | 0.53 | - |
isovaleryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
| 1.3.8.4 | 0.64 | - |
hexanoyl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
| 1.3.8.4 | 0.72 | - |
isovaleryl-CoA as substrate and 0.012 mM electron transfer flavoprotein as acceptor | Homo sapiens |
| 1.3.8.4 | 1.09 | - |
valeryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
| 1.3.8.4 | 2.91 | - |
isovaleryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
| 1.3.8.7 | 6.75 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.8.1 | butyryl-CoA + electron acceptor | intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | hexanoyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | - |
Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | pentanoyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | pentanoyl-CoA + phenazine methosulfate | pentanoyl-CoA i.e. valeryl-CoA | Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | propionyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-propenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.1 | propionyl-CoA + phenazine methosulfate | - |
Homo sapiens | 2-propenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.4 | butyryl-CoA + acceptor | acceptor: phenazine methosulfate | Homo sapiens | but-2-enoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.4 | hexanoyl-CoA + acceptor | acceptor: phenazine methosulfate | Homo sapiens | hex-2-enoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.4 | isovaleryl-CoA + acceptor | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.4 | isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.4 | isovaleryl-CoA + electron-transfer flavoprotein | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein | - |
r | |
| 1.3.8.4 | isovaleryl-CoA + phenazine methosulfate | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced phenazine methosulfate | - |
? | |
| 1.3.8.7 | acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | acyl-CoA + acceptor | chain-length specificity depending on electron acceptor: with phenazine methosulfate hexanoyl-CoA, with electron transfer flavoprotein, octanoyl-CoA | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | butanoyl-CoA + acceptor | - |
Homo sapiens | crotonyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | decanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-decenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | dodecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-dodecenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | hexadecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, very low activity | Homo sapiens | 2-hexadecenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | hexanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, best substrate with phenazine methosulfate | Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate with electron transferring flavoprotein | Homo sapiens | 2-octenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | octadecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, extremely low activity | Homo sapiens | 2-octadecenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | pentanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | tetradecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, low activity | Homo sapiens | 2-tetradecenoyl-CoA + reduced acceptor | - |
? | |
| 1.3.8.7 | tetradecenoyl-CoA + reduced electron transfer flavoprotein | - |
Homo sapiens | tetradecanoyl-CoA + electron transfer flavoprotein | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.8.1 | tetramer | 4 * 41200, SDS-PAGE | Homo sapiens |
| 1.3.8.4 | tetramer | 4 * 42000, SDS-PAGE | Homo sapiens |
| 1.3.8.7 | tetramer | 4 * 44000, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.8.1 | 16.5 | - |
hexanoyl-CoA | - |
Homo sapiens | |
| 1.3.8.1 | 20.8 | - |
butyryl-CoA | - |
Homo sapiens | |
| 1.3.8.1 | 40.7 | - |
valeryl-CoA | - |
Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.8.1 | 8 | - |
- |
Homo sapiens |
| 1.3.8.4 | 8 | - |
isovaleryl-CoA + phenazine methosulfate | Homo sapiens |
| 1.3.8.7 | 8 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.8.1 | 6.5 | 9 | less than 50% of maximal activity above and below | Homo sapiens |
| 1.3.8.7 | 6 | 9.5 | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.8.1 | FAD | 1 mol per mol of subunit | Homo sapiens | |
| 1.3.8.4 | FAD | 1 mol of FAD per subunit | Homo sapiens | |
| 1.3.8.7 | FAD | - |
Homo sapiens | |
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