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Literature summary extracted from
- Purification and properties of an extreme thermostable glutamate dehydrogenase from the archaebacterium Sulfolobus solfataricus (1991), Biochim. Biophys. Acta, 1073, 142-148.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.4.1.3 | SDS: 0.1% v/v, no loss of activity after 12 h, 0.5%, half-life 5 h | Saccharolobus solfataricus |
| 1.4.1.3 | urea: 4 M, 10% loss of activity after 12 h, 7.5 M, half-life 9 h | Saccharolobus solfataricus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.3 | D-glutamate | 2 mM, 11% inhibition, 6 mM, 34% inhibition | Saccharolobus solfataricus |  |
| 1.4.1.3 | pyridoxal 5'-phosphate | 2 mM, complete loss of activity | Saccharolobus solfataricus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.3 | 0.007 | - |
NADH | + 2-oxoglutarate | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.01 | - |
NADPH | + 2-oxoglutarate | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.025 | - |
NADP+ | + L-glutamate | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.063 | - |
NAD+ | + L-glutamate | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.2 | - |
2-oxoglutarate | cosubstrate NADPH | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.3 | - |
L-glutamate | cosubstrate NAD+ | Saccharolobus solfataricus | |
| 1.4.1.3 | 0.6 | - |
2-oxoglutarate | cosubstrate NADH | Saccharolobus solfataricus | |
| 1.4.1.3 | 1.1 | - |
L-glutamate | cosubstrate NADP+ | Saccharolobus solfataricus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 44000 | - |
6 * 44000, SDS-PAGE | Saccharolobus solfataricus |
| 1.4.1.3 | 270000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.4.1.3 | Ethanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
| 1.4.1.3 | isopropanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
| 1.4.1.3 | Methanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.3 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.3 | - |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 423 | - |
at 70°C | Saccharolobus solfataricus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.1.3 | -70°C, stable for 6 months | Saccharolobus solfataricus |
| 1.4.1.3 | 4°C, 20% loss of activity after 40 days | Saccharolobus solfataricus |
| 1.4.1.3 | 4°C, more than 20 days, no loss of activity | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.3 | 2-oxoglutarate + NH3 + NADH | - |
Saccharolobus solfataricus | L-glutamate + H2O + NAD+ | - |
? | |
| 1.4.1.3 | L-glutamate + H2O + NAD(P)+ | highly specific for 2-oxoglutarate and glutamate | Saccharolobus solfataricus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.3 | hexamer | 6 * 44000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 85 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 30 | 90 | 30°C: about 90% of activity maximum, 90°C: about 35% of activity maximum | Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 70 | 100 | no loss in activity after 1 h at 90°C, 20% residual activity after 1 h at 100°C | Saccharolobus solfataricus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 7.6 | - |
NAD(P)H + 2-oxoglutarate + NH3 | Saccharolobus solfataricus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 7 | 8 | 90-100% activity | Saccharolobus solfataricus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.3 | 3 | 9 | at least 65% residual activity after 14 h | Saccharolobus solfataricus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.3 | NADH | - |
Saccharolobus solfataricus | |
| 1.4.1.3 | NADPH | - |
Saccharolobus solfataricus | |
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