EC Number | General Stability | Organism |
---|---|---|
1.4.3.3 | 2-mercaptoethanol stabilizes | Rhodotorula toruloides |
1.4.3.3 | changes in ionic strength, I: 16-82 mM, have only moderate effect on enzyme stability, stability maximum is 50 mM | Rhodotorula toruloides |
1.4.3.3 | glycerol, 10%, stabilizes | Rhodotorula toruloides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | 1,3-Butadien 1-carboxylic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 2-hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 2-hydroxybutyric acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 2-ketobutyric acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 3-Bromobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 3-chlorobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 3-Hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 3-methylbenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 3-Nitrobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | 4-hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | benzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | crotonic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | kojic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | nicotinic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | Picolinic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
1.4.3.3 | trans-2-Pentenoic acid | D-phenylglycine oxidation | Rhodotorula toruloides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 0.18 | - |
O2 | with D-methionine | Rhodotorula toruloides | |
1.4.3.3 | 0.29 | - |
D-phenylalanine | - |
Rhodotorula toruloides | |
1.4.3.3 | 0.3 | - |
D-tryptophan | - |
Rhodotorula toruloides | |
1.4.3.3 | 0.39 | - |
D-norleucine | - |
Rhodotorula toruloides | |
1.4.3.3 | 0.5 | - |
D-leucine | - |
Rhodotorula toruloides | |
1.4.3.3 | 0.83 | - |
D-methionine | - |
Rhodotorula toruloides | |
1.4.3.3 | 0.89 | - |
D-Norvaline | - |
Rhodotorula toruloides | |
1.4.3.3 | 13.75 | - |
D-serine | - |
Rhodotorula toruloides | |
1.4.3.3 | 18.89 | - |
D-valine | - |
Rhodotorula toruloides | |
1.4.3.3 | 21.41 | - |
D-cysteine | - |
Rhodotorula toruloides | |
1.4.3.3 | 21.47 | - |
D-proline | - |
Rhodotorula toruloides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | D-alanine + H2O + O2 | Rhodotorula toruloides | - |
pyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-cysteine + H2O + O2 | Rhodotorula toruloides | - |
2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-leucine + H2O + O2 | Rhodotorula toruloides | - |
4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-methionine + H2O + O2 | Rhodotorula toruloides | - |
4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-norleucine + H2O + O2 | Rhodotorula toruloides | - |
2-oxohexanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-norvaline + H2O + O2 | Rhodotorula toruloides | - |
2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-phenylalanine + H2O + O2 | Rhodotorula toruloides | - |
phenylpyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-proline + H2O + O2 | Rhodotorula toruloides | - |
2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-serine + H2O + O2 | Rhodotorula toruloides | - |
2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-tryptophan + H2O + O2 | Rhodotorula toruloides | - |
indol-3-pyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-valine + H2O + O2 | Rhodotorula toruloides | - |
alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | thiazolidine-2-carboxylic acid + H2O + O2 | Rhodotorula toruloides | - |
? + NH3 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Rhodotorula toruloides | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.4.3.3 | a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2 | ping pong bi bi mechanism | Rhodotorula toruloides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-cysteine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-leucine + H2O + O2 | - |
Rhodotorula toruloides | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-methionine + H2O + O2 | - |
Rhodotorula toruloides | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-norleucine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxohexanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-norvaline + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-proline + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-serine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-tryptophan + H2O + O2 | - |
Rhodotorula toruloides | indol-3-pyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-valine + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | thiazolidine-2-carboxylic acid + H2O + O2 | - |
Rhodotorula toruloides | ? + NH3 + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 40 | 45 | - |
Rhodotorula toruloides |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 30 | 50 | less than 50% of maximal activity above and below | Rhodotorula toruloides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 523 | - |
D-valine | - |
Rhodotorula toruloides | |
1.4.3.3 | 721 | - |
D-alanine | - |
Rhodotorula toruloides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 8 | 8.5 | - |
Rhodotorula toruloides |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 7.3 | 7.9 | 30 min stable | Rhodotorula toruloides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | FAD | flavoprotein | Rhodotorula toruloides |