EC Number | General Stability | Organism |
---|---|---|
1.5.1.3 | freezing and thawing, significant loss of activity even in presence of glycerol | Glycine max |
1.5.1.3 | glycerol, 25%, essential for stabilization during purification | Glycine max |
1.5.1.3 | protease inhibitors phenylmethylsulfonyl fluoride and leupeptin are essential for stabilization during purification | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | 2,4-diaminoquinazoline | derivatives | Glycine max | |
1.5.1.3 | folate | - |
Glycine max | |
1.5.1.3 | K+ | - |
Glycine max | |
1.5.1.3 | methotrexate | - |
Glycine max | |
1.5.1.3 | pyrimethamine | - |
Glycine max | |
1.5.1.3 | trimethoprim | - |
Glycine max | |
1.5.1.3 | Urea | - |
Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.017 | - |
7,8-dihydrofolate | - |
Glycine max | |
1.5.1.3 | 0.03 | - |
NADPH | - |
Glycine max |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | organic mercurials | no activation | Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 22000 | - |
SDS-PAGE | Glycine max |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Glycine max | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.3 | seedling | - |
Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 15 | - |
crude extract | Glycine max |
1.5.1.3 | 56.5 | - |
purified enzyme | Glycine max |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.3 | 4°C, 0.1 M imidazole, pH 7.2, 25% glycerol, stable | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Glycine max | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | ? | ? * 22000, SDS-PAGE | Glycine max |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7.4 | - |
- |
Glycine max |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Glycine max |