EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | dithiothreitol | - |
Heliothis virescens | |
1.5.1.3 | p-chloromercuribenzoate | - |
Heliothis virescens |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.5.1.3 | medicine | potentially target for the discovery of novel insecticides and anthelminthics | Heliothis virescens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | amplification and DNA-sequencing of full length gene from DNA-library | Heliothis virescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | methotrexate | complete inhibition at 0.0001 mM, slow tight binding inhibitor | Heliothis virescens | |
1.5.1.3 | p-hydroxymercuribenzoate | - |
Heliothis virescens | |
1.5.1.3 | pyrimethamine | anti-malarial DHFR inhibitor | Heliothis virescens | |
1.5.1.3 | Sodium tetrathionate | thiol-blocking reagent | Heliothis virescens | |
1.5.1.3 | trimethoprim | - |
Heliothis virescens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.3 | soluble | - |
Drosophila melanogaster | - |
- |
1.5.1.3 | soluble | at low levels in the cytosol | Heliothis virescens | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 19000 | 21000 | DNA-sequence dtermination and SDS-PAGE | Heliothis virescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Drosophila melanogaster | - |
- |
- |
1.5.1.3 | Heliothis virescens | - |
i.e. Helicoverpa virescens, tobacco budworm | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Drosophila melanogaster |
1.5.1.3 | - |
Heliothis virescens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.1.3 | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | substrate and antifolate inhibitor methotrexate binding, modeling of three-dimensional structure | Heliothis virescens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.3 | larva | - |
Heliothis virescens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 20.8 | - |
purified enzyme | Heliothis virescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | strongly specific for | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH | strongly specific for | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | folate + NADPH | no activity | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | folate + NADPH | no activity | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | specific | Drosophila melanogaster | |
1.5.1.3 | NADPH | specific | Heliothis virescens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0000003 | - |
methotrexate | - |
Heliothis virescens | |
1.5.1.3 | 0.000021 | - |
pyrimethamine | - |
Heliothis virescens | |
1.5.1.3 | 0.00024 | - |
trimethoprim | - |
Heliothis virescens |