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Literature summary extracted from

  • Graham, D.E.; Graupner, M.; Xu, H.; White, R.H.
    Identification of coenzyme M biosynthetic 2-phosphosulfolactate phosphatase. A member of a new class of Mg2+-dependent acid phosphatases (2001), Eur. J. Biochem., 268, 5176-5188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.71 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.71 2-carboxyethylphosphonate weak, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Ca2+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Cd2+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Cu+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Fe2+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Fe3+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 Mg2+ in presence of equimolar Mg2+, polyhistidine-tagged enzyme Methanocaldococcus jannaschii
3.1.3.71 vanadate
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.71 0.009
-
 (S)-phosphomalate
-
 Methanocaldococcus jannaschii
3.1.3.71 0.011
-
 (R)-phosphomalate
-
 Methanocaldococcus jannaschii
3.1.3.71 0.034
-
 Phosphoglycolate
-
 Methanocaldococcus jannaschii
3.1.3.71 0.063
-
 rac-phosphosulfolactate
-
 Methanocaldococcus jannaschii
3.1.3.71 0.148
-
 (S)-phospholactate
-
 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.71 Co2+ can fully substitute for Mg2+ Methanocaldococcus jannaschii
3.1.3.71 Cu2+ weak activation Methanocaldococcus jannaschii
3.1.3.71 Mg2+ dependent on, at a concentration approximately equimolar with substrate Methanocaldococcus jannaschii
3.1.3.71 Mn2+ can fully substitute for Mg2+ Methanocaldococcus jannaschii
3.1.3.71 Ni2+ can fully substitute for Mg2+ Methanocaldococcus jannaschii
3.1.3.71 Zn2+ can fully substitute for Mg2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.71 30000
-
 x * 30000, SDS-PAGE Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.71 2-phospho-3-sulfolactate + H2O Methanocaldococcus jannaschii the enzyme catalyzes the second step in biosynthesis of coenzyme M 3-sulfolactate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.71 Methanocaldococcus jannaschii
-
-
-
3.1.3.71 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.71
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.71 48
-
 rac-phosphosulfolactate Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
3.1.3.71 -30°C, in presence of 50% v/v glycerol, 25% loss of activity after 3 weeks Methanocaldococcus jannaschii
3.1.3.71 4°C, 7% loss of activity after 3 weeks Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.71 (R)-2-phospholactate + H2O
-
 Methanocaldococcus jannaschii lactate + phosphate
-
 ?
3.1.3.71 (R)-phosphomalate + H2O
-
 Methanocaldococcus jannaschii malate + phosphate
-
 ?
3.1.3.71 (S)-2-phospholactate + H2O no activity with (R)-phospholactate Methanocaldococcus jannaschii lactate + phosphate
-
 ?
3.1.3.71 (S)-2-phosphomalate + H2O
-
 Methanocaldococcus jannaschii malate + phosphate
-
 ?
3.1.3.71 2-phospho-3-sulfolactate + H2O the enzyme catalyzes the second step in biosynthesis of coenzyme M Methanocaldococcus jannaschii 3-sulfolactate + phosphate
-
 ?
3.1.3.71 additional information in presence of (S)-lactate the histidine-tagged enzyme can dephosphorylate phosphoglycolate and produce 2-phospholactate Methanocaldococcus jannaschii ?
-
 ?
3.1.3.71 phosphoglycolate + H2O
-
 Methanocaldococcus jannaschii glycolate + phosphate
-
 ?
3.1.3.71 rac-2-phosphosulfolactate + H2O
-
 Methanocaldococcus jannaschii sulfolactate + phosphate
-
 ?
3.1.3.71 rac-2-phosphosulfolactate + H2O
-
 Methanosarcina thermophila sulfolactate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.71 ? x * 30000, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.71 75
-
 polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.71 65 85 65°C: about 50% of maximal activity, 85°C: about 70% of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.71 5.5
-
 polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.71 4.5 7 pH 4.5: about 80% of maximal activity, pH 7.0: about 55%of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate Methanocaldococcus jannaschii