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Literature summary extracted from

  • Bronnenmeier, K.; Rucknagel, K.P.; Staudenbauer, W.L.
    Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium (1991), Eur. J. Biochem., 200, 379-385.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.74 extracellular
-
 Thermoclostridium stercorarium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.74 87000
-
 x * 87000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.91 87000
-
 x * 87000, SDS-PAGE Thermoclostridium stercorarium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Thermoclostridium stercorarium
-
-
-
3.2.1.91 Thermoclostridium stercorarium P50900
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 12fold Thermoclostridium stercorarium
3.2.1.91 avicellase II Thermoclostridium stercorarium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.74 culture medium
-
 Thermoclostridium stercorarium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.74 0.0037
-
-
 Thermoclostridium stercorarium
3.2.1.74 0.025
-
 cellulose MN300 Thermoclostridium stercorarium
3.2.1.74 0.03
-
 cellulose HBS Thermoclostridium stercorarium
3.2.1.74 0.041
-
 avicel Thermoclostridium stercorarium
3.2.1.74 0.102
-
 H3PO4 swollen avicel Thermoclostridium stercorarium
3.2.1.74 0.108
-
 HCl swollen avicel Thermoclostridium stercorarium
3.2.1.74 0.128
-
 celloheptaose Thermoclostridium stercorarium
3.2.1.74 0.236
-
 cellohexaose Thermoclostridium stercorarium
3.2.1.74 0.34
-
 xylan Thermoclostridium stercorarium
3.2.1.74 0.42
-
 cellopentaose Thermoclostridium stercorarium
3.2.1.74 0.684
-
 cellotetraose Thermoclostridium stercorarium
3.2.1.91 0.037
-
-
 Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 Avicel + H2O H3PO4 or HCl swollen Thermoclostridium stercorarium D-glucose + cellotetraose + cellotriose + cellobiose cellobiose and cellotriose only ?
3.2.1.74 celloheptaose + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
3.2.1.74 cellohexaose + H2O
-
 Thermoclostridium stercorarium D-glucose + ? cellotetraose, cellotriose and cellobiose only ?
3.2.1.74 cellopentaose + H2O
-
 Thermoclostridium stercorarium D-glucose + ? cellobiose and cellotriose only ?
3.2.1.74 cellotetraose + H2O
-
 Thermoclostridium stercorarium D-glucose + cellotriose + cellobiose cellobiose only ?
3.2.1.74 cellulose + H2O cellulose HBS and cellulose MN300 Thermoclostridium stercorarium D-glucose + ?
-
 ?
3.2.1.74 xylan + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
3.2.1.91 avicel + H2O
-
 Thermoclostridium stercorarium cellobiose
-
 ?
3.2.1.91 carboxymethylcellulose + H2O
-
 Thermoclostridium stercorarium cellobiose + ?
-
 ?
3.2.1.91 cellotetraose + H2O
-
 Thermoclostridium stercorarium cellobiose + D-glucose + cellotriose
-
 ?
3.2.1.91 cellulose + H2O
-
 Thermoclostridium stercorarium cellobiose + D-glucose + cellotriose
-
 ?
3.2.1.91 xylan + H2O
-
 Thermoclostridium stercorarium xylobiose + xylotetraose + xylohexaose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.74 ? x * 87000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.91 ? x * 87000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.74 70
-
-
 Thermoclostridium stercorarium
3.2.1.91 70
-
-
 Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 5 6
-
 Thermoclostridium stercorarium
3.2.1.91 5 6
-
 Thermoclostridium stercorarium