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Literature summary extracted from

  • Beer, N.S.; Griffiths, W.T.
    Purification of the enzyme NADPH: protochlorophyllide oxidoreductase (1981), Biochem. J., 195, 83-92.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 etioplast
-
 Phaseolus vulgaris 9513
-
1.3.1.33 etioplast
-
 Hordeum vulgare 9513
-
1.3.1.33 etioplast
-
 Avena sativa 9513
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.33 35000
-
-
 Phaseolus vulgaris
1.3.1.33 35000
-
 gel filtration Hordeum vulgare
1.3.1.33 35000
-
 gel filtration Avena sativa
1.3.1.33 35000
-
 SDS-PAGE shows 2 closely related peptides of 35000 and 37000 Avena sativa
1.3.1.33 37000
-
-
 Hordeum vulgare
1.3.1.33 37000
-
 gel filtration, doublet of 2 peptides of 35000 and 37000 co-purified Avena sativa
1.3.1.33 38000
-
 gel filtration Hordeum vulgare
1.3.1.33 45000
-
 2 * 45000, SDS-PAGE Phaseolus vulgaris
1.3.1.33 63000
-
-
 Hordeum vulgare
1.3.1.33 100000
-
-
 Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Phaseolus vulgaris
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Hordeum vulgare
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Avena sativa
-
 chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Avena sativa
-
 oat
-
1.3.1.33 Avena sativa
-
 L., var. Pennal
-
1.3.1.33 Hordeum vulgare
-
 barley
-
1.3.1.33 Hordeum vulgare
-
 L., cultivar Proctor
-
1.3.1.33 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33
-
 Hordeum vulgare
1.3.1.33
-
 Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.33 leaf
-
 Phaseolus vulgaris
-
1.3.1.33 leaf
-
 Hordeum vulgare
-
1.3.1.33 leaf
-
 Avena sativa
-
1.3.1.33 shoot
-
 Hordeum vulgare
-
1.3.1.33 shoot
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.33 0.5
-
 etioplast membranes Hordeum vulgare
1.3.1.33 13.9
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
 Phaseolus vulgaris chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Hordeum vulgare chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Avena sativa chlorophyllide + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.33 dimer 2 * 45000, SDS-PAGE Phaseolus vulgaris