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Literature summary extracted from

  • Giuditta, A.; Strecker, H.J.
    Purification and some properties of a brain diaphorase (1961), Biochim. Biophys. Acta, 48, 10-19.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 1,10-phenanthroline
-
 Bos taurus
1.6.5.2 2,4-dinitrophenol 0.067 mM, 56% inhibition Bos taurus
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin) 0.000033 mM, 56% inhibition Bos taurus
1.6.5.2 4-hydroxymercuribenzoate 0.067 mM, 50% inhibition Bos taurus
1.6.5.2 Amytal 6.6 mM, 55% inhibition Bos taurus
1.6.5.2 N-ethyl maleimide 0.33 mM, complete inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0025
-
 menadione cofactor NADH Bos taurus
1.6.5.2 0.024
-
 methylene blue cofactor NADPH Bos taurus
1.6.5.2 0.04
-
 menadione cofactor NADPH Bos taurus
1.6.5.2 0.3
-
 vitamin K1 cofactor NADPH Bos taurus
1.6.5.2 0.33
-
 vitamin K1 cofactor NADH Bos taurus
1.6.5.2 1.4
-
 potassium ferricyanide cofactor NADPH Bos taurus
1.6.5.2 1.7
-
 potassium ferricyanide cofactor NADH Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 additional information
-
 230000.0 units/mg, 1 unit is defined as the amount of enzyme causing a decrease in absorbance at 340 nm of 0.001/min Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Bos taurus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 2-methyl-3-phytyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2-methyl-3-phytyl-1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 K3[Fe(CN)6] + NAD(P)H no activity with coenzyme Q10 Bos taurus K4[Fe(CN)6] + NAD(P)+
-
 ?
1.6.5.2 K3[Fe(CN)6] + NAD(P)H no activity with lipoic acid, cytochrome c and vitamin K1 Bos taurus K4[Fe(CN)6] + NAD(P)+
-
 ?
1.6.5.2 methylene blue + NAD(P)H
-
 Bos taurus reduced methylene blue + NAD(P)+
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Bos taurus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.5.2 potassiumferricyanide + NADH + H+
-
 Bos taurus potassium ferrocyanide + NAD+
-
 r
1.6.5.2 potassiumferricyanide + NADPH + H+
-
 Bos taurus potassium ferrocyanide + NADP+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD stimulates Bos taurus
1.6.5.2 FAD enzyme contains FAD Bos taurus
1.6.5.2 FMN stimulation Bos taurus
1.6.5.2 NAD+
-
 Bos taurus
1.6.5.2 NADP+
-
 Bos taurus