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Literature summary extracted from

  • Luan, P.; Arechaga-Ocampo, E.; Sarath, G.; Arredondo-Peter, R.; Klucas, R.V.
    Analysis of a ferric leghemoglobin reductase from cowpea (Vigna unguiculata) root nodules (2000), Plant Sci., 154, 161-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.6 cloning of cDNA Vigna unguiculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.6 0.0104
-
 ferrileghemoglobin cowpea ferrileghemoglobin Vigna unguiculata
1.6.2.6 0.0124
-
 ferrileghemoglobin soybean ferrileghemoglobin Vigna unguiculata
1.6.2.6 0.057
-
 NADH
-
 Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.6 55000
-
 2 * 55000, SDS-PAGE Vigna unguiculata
1.6.2.6 56000
-
 2 * 56000, deduced from amino acid sequence Vigna unguiculata
1.6.2.6 110000
-
 gel filtration Vigna unguiculata

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.6 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.6 ammonium sulfate, hydroxylapatite, ion exchange, gel filtration Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.6 root nodule
-
 Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.6 0.216
-
 reduction of ferrileghemoglobin Vigna unguiculata
1.6.2.6 2.565
-
 reduction of 2,6-dichloroindophenol Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.6 2,6-dichloroindophenol + NADH
-
 Vigna unguiculata reduced 2,6-dichloroindophenol + NAD+
-
 ?
1.6.2.6 NAD(P)H + ferrileghemoglobin reduces both cowpea ferrihemoglobin and soybean ferrileghemoglobin Vigna unguiculata NAD(P)+ + ferroleghemoglobin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.6 dimer 2 * 55000, SDS-PAGE Vigna unguiculata
1.6.2.6 dimer 2 * 56000, deduced from amino acid sequence Vigna unguiculata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.6 2.5
-
 ferrileghemoglobin soybean ferrileghemoglobin Vigna unguiculata
1.6.2.6 3.1
-
 ferrileghemoglobin cowpea ferrileghemoglobin Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.6 6.5
-
 reduction of ferrileghemoglobin Vigna unguiculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.6 4.8 8.5 no activity below and above Vigna unguiculata