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Literature summary extracted from
- Di-fluoresceinthiocarbamyl-insulin: A fluorescent substrate for the assay of protein disulfide oxidoreductase activity (1997), Anal. Biochem., 248, 94-101.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | Ca2+ | no inhibition | Brassica napus |  |
| 1.8.4.2 | Mg2+ | no inhibition | Brassica napus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.2 | Brassica napus | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.4.2 | 2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | mechanism | Brassica napus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | additional information | - |
fluorometric assay method | Brassica napus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.2 | dithiothreitol + protein disulfide | protein disulfide: insulin or thioredoxin | Brassica napus | ? | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | 7.9 | - |
thioredoxin | Brassica napus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | 6.2 | 8.4 | - |
Brassica napus |
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Release 2023.1 (January 2023)
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