EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.99.5 | KCN | 1 mM, complete inhibition | Clostridium pasteurianum | |
1.8.99.5 | additional information | not inhibitory: iodoacetate | Clostridium pasteurianum | |
1.8.99.5 | NaN3 | 1 mM, 18% inhibition | Clostridium pasteurianum | |
1.8.99.5 | Tris/HCl | Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5 | Clostridium pasteurianum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.99.5 | 0.35 | - |
sulfite | pH 7.5, 37°C | Clostridium pasteurianum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.99.5 | 13400 | - |
2 * 13400 plus 2 * 28400, SDS-PAGE | Clostridium pasteurianum |
1.8.99.5 | 28400 | - |
2 * 13400 plus 2 * 28400, SDS-PAGE | Clostridium pasteurianum |
1.8.99.5 | 83600 | - |
PAGE | Clostridium pasteurianum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.99.5 | Clostridium pasteurianum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.5 | hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+ | - |
Clostridium pasteurianum | ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O | 50% of the activity with sulfite | ? | |
1.8.99.5 | additional information | no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1 | Clostridium pasteurianum | ? | - |
? | |
1.8.99.5 | nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor | - |
Clostridium pasteurianum | ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O | 25% of the activity with sulfite | ? | |
1.8.99.5 | SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+ | - |
Clostridium pasteurianum | HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O | 40% of the activity with sulfite | ? | |
1.8.99.5 | sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+ | - |
Clostridium pasteurianum | hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O | overall reaction | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.99.5 | heterotetramer | 2 * 13400 plus 2 * 28400, SDS-PAGE | Clostridium pasteurianum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.99.5 | 7.5 | - |
phosphate buffer | Clostridium pasteurianum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.99.5 | additional information | no activity is observed with NAD, NADP, FAD or FMN | Clostridium pasteurianum |