BRENDA - Enzyme Database

A thiol-disulfide transhydrogenase from yeast

Nagai, S.; Black, S.; J. Biol. Chem. 243, 1942-1947 (1968)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.8.4.4
ammonium sulfate, 1 M, complete stabilization, 6 days at 20°C
Saccharomyces cerevisiae
1.8.4.4
cystine, alone: no stabilization, in addition to glutathione: increases stabilizing effect
Saccharomyces cerevisiae
1.8.4.4
EDTA stabilizes
Saccharomyces cerevisiae
1.8.4.4
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
1.8.4.4
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
1.8.4.4
glycerol, no stabilization
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.4
cell associated
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.4.4
150000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.4
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.8.4.4
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.8.4.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.4
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
1.8.4.4
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.8.4.4
beta-hydroxyethyl disulfide + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
cystine + GSH
-
395014
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
1.8.4.4
D-cystine + 2 GSH
-
395014
Saccharomyces cerevisiae
2 D-cysteine + GSSG
-
-
-
?
1.8.4.4
diacetyl-L-cystine + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
homocystine + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
L-cystine + GSH
-
395014
Saccharomyces cerevisiae
L-cysteine + GSSG
-
-
-
?
1.8.4.4
L-cystine diamide + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
L-cystinyldiglycine + GSH
-
395014
Saccharomyces cerevisiae
L-Cys-Gly-Gly + GSSG
-
-
-
?
1.8.4.4
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395014
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.4
23
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.4
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.4.4
6
8.5
pH 6.0: 10% of activity maximum, pH 8.5: activity maximum
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
1.8.4.4
ammonium sulfate, 1 M, complete stabilization, 6 days at 20°C
Saccharomyces cerevisiae
1.8.4.4
cystine, alone: no stabilization, in addition to glutathione: increases stabilizing effect
Saccharomyces cerevisiae
1.8.4.4
EDTA stabilizes
Saccharomyces cerevisiae
1.8.4.4
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
1.8.4.4
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
1.8.4.4
glycerol, no stabilization
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.4
cell associated
-
Saccharomyces cerevisiae
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.4.4
150000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.4
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.4
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.4.4
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.8.4.4
beta-hydroxyethyl disulfide + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
cystine + GSH
-
395014
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
1.8.4.4
D-cystine + 2 GSH
-
395014
Saccharomyces cerevisiae
2 D-cysteine + GSSG
-
-
-
?
1.8.4.4
diacetyl-L-cystine + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
homocystine + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
L-cystine + GSH
-
395014
Saccharomyces cerevisiae
L-cysteine + GSSG
-
-
-
?
1.8.4.4
L-cystine diamide + GSH
-
395014
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
L-cystinyldiglycine + GSH
-
395014
Saccharomyces cerevisiae
L-Cys-Gly-Gly + GSSG
-
-
-
?
1.8.4.4
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395014
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.4
23
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.4
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.4.4
6
8.5
pH 6.0: 10% of activity maximum, pH 8.5: activity maximum
Saccharomyces cerevisiae