EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.98.1 | 0.002 | - |
H2 | + methyl viologen, pH 9 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 0.01 | - |
H2 | + coenzyme F0, pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 0.019 | - |
coenzyme F420 | pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 0.034 | - |
coenzyme F0 | pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 0.42 | - |
methyl viologen | pH 9 | Methanothermobacter thermautotrophicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | Iron | - |
Methanothermobacter thermautotrophicus | |
1.12.98.1 | sulfur | - |
Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 500000 | - |
native enzyme bigger than 500000 | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F420 | Methanothermobacter thermautotrophicus | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.98.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.98.1 | partial | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F0 | F0 is 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanothermobacter thermautotrophicus | reduced coenzyme F0 | - |
? | |
1.12.98.1 | H2 + coenzyme F420 | F420 is 7,8-didemethyl-8-hydroxy-5-deazaflavin | Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? | |
1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? | |
1.12.98.1 | H2 + methyl viologen | - |
Methanothermobacter thermautotrophicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.98.1 | multimer | alpha,beta,gamma 40000 + 31000 + 26000, SDS-PAGE, exact subunit composition not known, ratio 2:2:1 leading to MW 170000 | Methanothermobacter thermautotrophicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.98.1 | 725 | - |
methyl viologen | pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 725 | - |
coenzyme F420 | pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 725 | - |
coenzyme F0 | pH 7.2 | Methanothermobacter thermautotrophicus | |
1.12.98.1 | 4000 | - |
methyl viologen | pH 9 | Methanothermobacter thermautotrophicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | FAD | 2.3 mol per mol 170000 Da unit | Methanothermobacter thermautotrophicus |