EC Number | General Stability | Organism |
---|---|---|
1.3.1.24 | loss of activity in solutions with protein concentration below 0.030-0.035 mg/ml | Rattus norvegicus |
1.3.1.24 | NADPH stabilizes | Rattus norvegicus |
1.3.1.24 | repeated freeze-thawing: little loss of activity , no significant loss of activity, partially purified enzyme | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.24 | 5,5'-dithiobis(2-nitrobenzoate) | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
1.3.1.24 | bilirubin | inhibits the enzyme in a competitive manner | Rattus norvegicus | |
1.3.1.24 | Biliverdin | the enzyme is inhibited by the substrate when the concentration exceeds 0.004-0.005 mM | Rattus norvegicus | |
1.3.1.24 | Hematin | - |
Rattus norvegicus | |
1.3.1.24 | HgCl2 | irreversible inhibition | Rattus norvegicus | |
1.3.1.24 | iodoacetamide | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
1.3.1.24 | Metalloporphyrin complexes | - |
Rattus norvegicus | |
1.3.1.24 | N-ethylmaleimide | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
1.3.1.24 | p-chloromercuribenzoate | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
1.3.1.24 | SH-reagents | e.g. 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide, pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
1.3.1.24 | zinc-protoporphyrin | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.24 | 0.003 | - |
NADPH | - |
Rattus norvegicus | |
1.3.1.24 | 0.003 | - |
Biliverdin | cosubstrate NADPH | Rattus norvegicus | |
1.3.1.24 | 0.005 | - |
Biliverdin | cosubstrate NADH | Rattus norvegicus | |
1.3.1.24 | 0.27 | - |
NADH | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.24 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.24 | 34000 | 36000 | liver | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.24 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.24 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.24 | 3.5 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.24 | -20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity | Rattus norvegicus |
1.3.1.24 | 0-4°C, 5 days | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.24 | bilirubin + NAD+ | - |
Rattus norvegicus | biliverdin + NADH + H+ | - |
? | |
1.3.1.24 | biliverdin + NAD(P)H | - |
Rattus norvegicus | bilirubin + NAD(P)+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.24 | 45 | - |
10 min, no loss of activity | Rattus norvegicus |
1.3.1.24 | 55 | - |
10 min, complete loss of activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.24 | 7 | - |
biliverdin + NADH | Rattus norvegicus |
1.3.1.24 | 8.7 | - |
biliverdin + NADPH | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.24 | 7.4 | - |
0.1 M potassium phosphate buffer, most stable | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.24 | NADH | - |
Rattus norvegicus | |
1.3.1.24 | NADPH | - |
Rattus norvegicus |