BRENDA - Enzyme Database show

A cytochrome P-450 monooxygenase catalyzes the first step in the conversion of tabersonine to vindoline in Catharanthus roseus

St-Pierre, B.; De Luca, V.; Plant Physiol. 109, 131-139 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.103
clotrimazole
50% inhibition at 0.05 mM
Catharanthus roseus
1.14.14.103
CO
concentration dependent competitive inhibition, 77% inhibition at 90%
Catharanthus roseus
1.14.14.103
cytochrome c
50% inhibition at 0.001 mM
Catharanthus roseus
1.14.14.103
miconazole
50% inhibition at 0.3 mM
Catharanthus roseus
1.14.14.103
tabersonine
inhibition above 0.03 mM
Catharanthus roseus
1.14.14.103
tricliphane
50% inhibition at 0.5 mM
Catharanthus roseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.103
0.011
-
tabersonine
-
Catharanthus roseus
1.14.14.103
0.014
-
NADPH
-
Catharanthus roseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.103
endoplasmic reticulum
-
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
first step in vindoline boxynthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.103
Catharanthus roseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
bud
-
Catharanthus roseus
-
1.14.14.103
leaf
-
Catharanthus roseus
-
1.14.14.103
root
-
Catharanthus roseus
-
1.14.14.103
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
tabersonine + NADPH + H+ + O2
NADH is less active as substrate
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
438776
Catharanthus roseus
?
1.14.14.103
tabersonine + NADPH + H+ + O2
first step in vindoline boxynthesis
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.103
30
-
assay at
Catharanthus roseus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.103
7.5
-
phosphate buffer
Catharanthus roseus
1.14.14.103
8
-
Tris buffer
Catharanthus roseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.103
clotrimazole
50% inhibition at 0.05 mM
Catharanthus roseus
1.14.14.103
CO
concentration dependent competitive inhibition, 77% inhibition at 90%
Catharanthus roseus
1.14.14.103
cytochrome c
50% inhibition at 0.001 mM
Catharanthus roseus
1.14.14.103
miconazole
50% inhibition at 0.3 mM
Catharanthus roseus
1.14.14.103
tabersonine
inhibition above 0.03 mM
Catharanthus roseus
1.14.14.103
tricliphane
50% inhibition at 0.5 mM
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.103
0.011
-
tabersonine
-
Catharanthus roseus
1.14.14.103
0.014
-
NADPH
-
Catharanthus roseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.103
endoplasmic reticulum
-
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
first step in vindoline boxynthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
bud
-
Catharanthus roseus
-
1.14.14.103
leaf
-
Catharanthus roseus
-
1.14.14.103
root
-
Catharanthus roseus
-
1.14.14.103
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
tabersonine + NADPH + H+ + O2
NADH is less active as substrate
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
438776
Catharanthus roseus
?
1.14.14.103
tabersonine + NADPH + H+ + O2
first step in vindoline boxynthesis
438776
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.103
30
-
assay at
Catharanthus roseus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.103
7.5
-
phosphate buffer
Catharanthus roseus
1.14.14.103
8
-
Tris buffer
Catharanthus roseus