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Literature summary extracted from

  • Kukor, J.J.; Olsen, R.H.
    Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments (1996), Appl. Environ. Microbiol., 62, 1728-1740.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expression in Escherichia coli Pseudomonas sp.
1.13.11.2 expression in Escherichia coli Pseudomonas putida
1.13.11.2 expression in Escherichia coli Ralstonia pickettii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 additional information
-
 additional information Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains Pseudomonas fluorescens
1.13.11.2 additional information
-
 additional information Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains Pseudomonas sp.
1.13.11.2 additional information
-
 additional information Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains Pseudomonas putida
1.13.11.2 additional information
-
 additional information Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains Burkholderia cepacia
1.13.11.2 additional information
-
 additional information Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains Ralstonia pickettii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Burkholderia cepacia
-
 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas fluorescens enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas sp. enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas putida enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Ralstonia pickettii enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas fluorescens CFS 215 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Ralstonia pickettii PK01 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Burkholderia cepacia G4
-
 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas sp. W31 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Burkholderia cepacia
-
-
-
1.13.11.2 Burkholderia cepacia G4
-
-
-
1.13.11.2 Pseudomonas fluorescens
-
-
-
1.13.11.2 Pseudomonas fluorescens CFS 215
-
-
-
1.13.11.2 Pseudomonas putida
-
 F1 and PaW1
-
1.13.11.2 Pseudomonas sp.
-
 W31
-
1.13.11.2 Pseudomonas sp. W31
-
 W31
-
1.13.11.2 Ralstonia pickettii
-
-
-
1.13.11.2 Ralstonia pickettii PK01
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.2 significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition Pseudomonas fluorescens
1.13.11.2 significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition Pseudomonas sp.
1.13.11.2 significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition Ralstonia pickettii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Pseudomonas fluorescens
1.13.11.2
-
 Pseudomonas sp.
1.13.11.2
-
 Ralstonia pickettii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas fluorescens 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Ralstonia pickettii 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas fluorescens CFS 215 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Ralstonia pickettii PK01 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Burkholderia cepacia G4 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas sp. W31 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Pseudomonas fluorescens 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Pseudomonas sp. 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Burkholderia cepacia 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Ralstonia pickettii 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Pseudomonas fluorescens CFS 215 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Ralstonia pickettii PK01 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Burkholderia cepacia G4 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 hypoxic strains with significantly higher affinities Pseudomonas sp. W31 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Burkholderia cepacia 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Pseudomonas fluorescens 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Burkholderia cepacia 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Ralstonia pickettii 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Pseudomonas fluorescens 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Ralstonia pickettii 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Pseudomonas fluorescens CFS 215 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Pseudomonas fluorescens CFS 215 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Ralstonia pickettii PK01 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Ralstonia pickettii PK01 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Burkholderia cepacia G4 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Burkholderia cepacia G4 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains Pseudomonas sp. W31 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring Pseudomonas sp. W31 2-hydroxymuconate semialdehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 additional information
-
 additional information the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains Pseudomonas fluorescens
1.13.11.2 additional information
-
 additional information the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains Pseudomonas sp.
1.13.11.2 additional information
-
 additional information the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains Pseudomonas putida
1.13.11.2 additional information
-
 additional information the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains Burkholderia cepacia
1.13.11.2 additional information
-
 additional information the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains Ralstonia pickettii