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Literature summary extracted from

  • Happe, R.P.; Roseboom, W.; Egert, G.; Friedrich, C.G.; Massanz, C.; Friedrich, B.; Albracht, S.P.
    Unusual FTIR and EPR properties of the H2-activating site of the cytoplasmic NAD-reducing hydrogenase from Ralstonia eutropha (2000), FEBS Lett., 466, 259-263.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Ni2+ presence of a Ni(CN)Fe(CN)3(CO) active site is suggested Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.2 23000
-
 1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000 Cupriavidus necator
1.12.1.2 26000
-
 1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000 Cupriavidus necator
1.12.1.2 55000
-
 1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000 Cupriavidus necator
1.12.1.2 67000
-
 1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000 Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 85
-
 electron acceptor NAD+ Cupriavidus necator
1.12.1.2 100
-
 electron acceptor benzyl viologen Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 2 ferricyanide + NAD(P)H
-
 Cupriavidus necator 2 ferrocyanide + NAD(P)+ + H+
-
 ?
1.12.1.2 H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.2 tetramer 1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000 Cupriavidus necator