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Literature summary extracted from
- Purification of a three-subunit ubiquinol-cytochrome c oxidoreductase complex from Paracoccus denitrificans [published erratum appears in J Biol Chem 1986 Nov 25;261(33):15813] (1986), J. Biol. Chem., 261, 12282-12289.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.8 | dodecyl maltoside | - |
Paracoccus denitrificans |  |
| 7.1.1.8 | dodecyl maltoside | - |
Bos taurus | |
| 7.1.1.8 | dodecyl maltoside | - |
Cereibacter sphaeroides | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.1.1.8 | slight loss of activity during purification | Paracoccus denitrificans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.8 | 2-Heptyl-4-hydroxyquinoline-N-oxide | - |
Bos taurus | |
| 7.1.1.8 | 2-Heptyl-4-hydroxyquinoline-N-oxide | - |
Paracoccus denitrificans | |
| 7.1.1.8 | 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole | - |
Bos taurus | |
| 7.1.1.8 | 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole | - |
Cereibacter sphaeroides | |
| 7.1.1.8 | 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole | - |
Paracoccus denitrificans | |
| 7.1.1.8 | Antimycin | - |
Bos taurus | |
| 7.1.1.8 | Antimycin | - |
Cereibacter sphaeroides | |
| 7.1.1.8 | Antimycin | - |
Paracoccus denitrificans | |
| 7.1.1.8 | Myxothiazol | - |
Bos taurus | |
| 7.1.1.8 | Myxothiazol | - |
Cereibacter sphaeroides | |
| 7.1.1.8 | Myxothiazol | - |
Paracoccus denitrificans | |
| 7.1.1.8 | Myxothiazol | - |
Spinacia oleracea | |
| 7.1.1.8 | Stigmatellin | - |
Bos taurus | |
| 7.1.1.8 | Stigmatellin | - |
Paracoccus denitrificans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.1.8 | membrane | - |
Bos taurus | 16020 | - |
| 7.1.1.8 | membrane | - |
Cereibacter sphaeroides | 16020 | - |
| 7.1.1.8 | membrane | plasma membrane | Paracoccus denitrificans | 16020 | - |
| 7.1.1.8 | mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.8 | Iron-sulfur cluster | - |
Bos taurus | |
| 7.1.1.8 | Iron-sulfur cluster | - |
Cereibacter sphaeroides | |
| 7.1.1.8 | Iron-sulfur cluster | - |
Spinacia oleracea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.8 | 20000 | - |
- |
Paracoccus denitrificans |
| 7.1.1.8 | 20000 | - |
Rieske iron sulfur protein | Bos taurus |
| 7.1.1.8 | 39000 | - |
cytochrome b | Paracoccus denitrificans |
| 7.1.1.8 | 60000 | 68000 | cytochrome c1 | Paracoccus denitrificans |
| 7.1.1.8 | 62000 | - |
- |
Bos taurus |
| 7.1.1.8 | 62000 | - |
cytochrome c1 | Paracoccus denitrificans |
| 7.1.1.8 | 121000 | - |
calculation from cytochrome content | Paracoccus denitrificans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.8 | Bos taurus | - |
- |
- |
| 7.1.1.8 | Cereibacter sphaeroides | - |
formerly Rhodopseudomonas sphaeroides | - |
| 7.1.1.8 | Paracoccus denitrificans | - |
- |
- |
| 7.1.1.8 | Spinacia oleracea | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 7.1.1.8 | phospholipoprotein | - |
Paracoccus denitrificans |
| 7.1.1.8 | phospholipoprotein | - |
Bos taurus |
| 7.1.1.8 | phospholipoprotein | - |
Cereibacter sphaeroides |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.8 | - |
Paracoccus denitrificans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.1.1.8 | -20°C, glycerol, completely stable for 6 months | Paracoccus denitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.8 | quinol + 2 ferricytochrome c | - |
Paracoccus denitrificans | quinone + 2 ferrocytochrome c + H+ [side 2] | - |
? | |
| 7.1.1.8 | ubiquinol + 2 ferricytochrome c | - |
Paracoccus denitrificans | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| 7.1.1.8 | ubiquinol + 2 ferricytochrome c | - |
Bos taurus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| 7.1.1.8 | ubiquinol + 2 ferricytochrome c | - |
Cereibacter sphaeroides | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| 7.1.1.8 | ubiquinol + 2 ferricytochrome c | - |
Spinacia oleracea | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.8 | pentamer | - |
Spinacia oleracea |
| 7.1.1.8 | trimer | - |
Cereibacter sphaeroides |
| 7.1.1.8 | trimer | 1 * 62000 + 1 * 39000 + 1 * 20000 | Paracoccus denitrificans |
| 7.1.1.8 | trimer | 1 * 62000 + 1 * 39000 + 1 * 20000, cytochrome c1, cytochrome b, Rieske-type iron-sulfur protein, SDS-PAGE | Paracoccus denitrificans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.8 | 7.83 | - |
2 ferricytochrome c | - |
Paracoccus denitrificans | |
| 7.1.1.8 | 13.3 | - |
cytochrome c | - |
Bos taurus | |
| 7.1.1.8 | 25 | 66.7 | cytochrome c | - |
Bos taurus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.8 | cytochrome b | - |
Bos taurus | |
| 7.1.1.8 | cytochrome b | - |
Cereibacter sphaeroides | |
| 7.1.1.8 | cytochrome c1 | - |
Bos taurus | |
| 7.1.1.8 | cytochrome c1 | - |
Cereibacter sphaeroides | |
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