EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.274 | F22Y/A272G | isoenzyme A, reduced kcat for NADH | Corynebacterium sp. |
1.1.1.274 | F22Y/K232G/R235G/R238E/A272G | isoenzyme A, increase in kcat for NADH | Corynebacterium sp. |
1.1.1.274 | F22Y/K232G/R235G/R238H/A272G | isoenzyme A, increase in kcat for NADH | Corynebacterium sp. |
1.1.1.274 | F22Y/K232G/R235T/R238E/A272G | isoenzyme A, 24fold increase in kcat for NADH | Corynebacterium sp. |
1.1.1.274 | F22Y/K232G/R238H/A272G | isoenzyme A, increase in kcat forNADH | Corynebacterium sp. |
1.1.1.274 | F22Y/S232T/R235S/R238H/A272G | isoenzyme A, increase in kcat for NADH | Corynebacterium sp. |
1.1.1.274 | K232G/R238H | isoenzyme A, increase in kcat for NADH | Corynebacterium sp. |
1.1.1.346 | F22Y/A272G | substrate-binding pocket double mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
1.1.1.346 | F22Y/K232G/R235G/R238H/A272G | mutant with wild type kcat value for NADPH | Corynebacterium sp. |
1.1.1.346 | F22Y/K232G/R235T/R238H/A272G 420 | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
1.1.1.346 | F22Y/K232G/R238H/A272G | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
1.1.1.346 | F22Y/K232G/R238H/A272G | the mutant exhibits activity with NADH that is more than 2 orders of magnitude higher than that of the wild type enzyme and retains a high level of activity with NADPH | Corynebacterium sp. |
1.1.1.346 | K232G/R238H | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.274 | 0.039 | - |
NADPH | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.055 | - |
NADPH | F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.058 | - |
NADPH | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.071 | - |
NADPH | F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.13 | - |
NADPH | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.15 | - |
NADPH | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.39 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 0.66 | - |
NADH | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 1.2 | - |
NADH | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 1.4 | - |
NADH | isoenzyme A, wild-type | Corynebacterium sp. | |
1.1.1.274 | 2.4 | - |
NADH | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 2.7 | - |
NADH | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 3.4 | - |
NADH | F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 3.9 | - |
NADH | F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 5.2 | - |
NADH | F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 7.2 | - |
2,5-didehydro-D-gluconate | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 8.7 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 9.1 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 13 | - |
2,5-didehydro-D-gluconate | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 13 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 27 | - |
2,5-didehydro-D-gluconate | F22Y/S232T/R235S/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 30 | - |
2,5-didehydro-D-gluconate | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 32 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 32 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 43 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 110 | - |
2,5-didehydro-D-gluconate | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 130 | - |
2,5-didehydro-D-gluconate | isoenzyme A, wild-type | Corynebacterium sp. | |
1.1.1.274 | 150 | - |
2,5-didehydro-D-gluconate | F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant | Corynebacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.274 | Corynebacterium sp. | - |
- |
- |
1.1.1.346 | Corynebacterium sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.274 | 2,5-didehydro-D-gluconate + NADH | - |
Corynebacterium sp. | 2-keto-L-gulonate + NAD+ | - |
? | |
1.1.1.346 | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.346 | 2,5-diketo-D-gluconic acid reductase | - |
Corynebacterium sp. |
1.1.1.346 | 2,5-DKG reductase | - |
Corynebacterium sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.274 | 1.23 | - |
NADH | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 2.17 | - |
NADPH | F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 2.83 | - |
NADH | isoenzyme A, wild-type | Corynebacterium sp. | |
1.1.1.274 | 3.33 | - |
NADPH | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 7 | - |
NADPH | F22Y/K232G/R235T/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 7.33 | - |
NADPH | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 8.5 | - |
NADH | F22Y/S233T/R235S/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 10 | - |
NADH | F22Y/K232G/R235G/R238E/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 16.5 | - |
NADH | F22Y/K232G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 18.3 | - |
NADPH | F22Y/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 18.3 | - |
NADPH | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 18.3 | - |
NADH | F22Y/K232G/R235G/R238H/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 20 | - |
NADH | K232G/R238H isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.274 | 68.3 | - |
NADH | F22Y/K232G/R235T/R238E/A272G isoenzyme A mutant | Corynebacterium sp. | |
1.1.1.346 | 2.2 | - |
NADPH | mutant enzyme F22Y/S233T/R235S/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
1.1.1.346 | 3.3 | - |
NADPH | mutant enzyme F22Y/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
1.1.1.346 | 7 | - |
NADPH | mutant enzyme F22Y/K232G/R235G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
1.1.1.346 | 7.3 | - |
NADPH | mutant enzyme K232G/R238H, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
1.1.1.346 | 18.3 | - |
NADPH | mutant enzyme F22Y/K232G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
1.1.1.346 | 18.3 | - |
NADPH | wild type enzyme, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.346 | NADPH | the enzyme has a preference for NADPH over NADH | Corynebacterium sp. |