EC Number | General Stability | Organism |
---|---|---|
1.14.20.1 | increasing ionic strength to 100 decreases stability of the enzyme | Amycolatopsis lactamdurans |
1.14.20.1 | the enzyme is very unstable and can be partially stabilized in 25 mM Tris-HCl, pH 9.0, in the presence of 0.1 mM DTT | Amycolatopsis lactamdurans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.20.1 | 2-oxobutyrate | 0.1 mM, complete inhibition | Amycolatopsis lactamdurans | |
1.14.20.1 | 3-oxoadipate | 0.1 mM, 56% inhibition | Amycolatopsis lactamdurans | |
1.14.20.1 | Co2+ | 1 mM, 79% inhibition | Amycolatopsis lactamdurans | |
1.14.20.1 | Cu2+ | 1 mM, complete inhibition | Amycolatopsis lactamdurans | |
1.14.20.1 | phosphate | - |
Amycolatopsis lactamdurans | |
1.14.20.1 | Zn2+ | 1 mM, 84% inhibition | Amycolatopsis lactamdurans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.1 | 0.003 | - |
oxoglutarate | - |
Amycolatopsis lactamdurans | |
1.14.20.1 | 0.052 | - |
Penicillin N | - |
Amycolatopsis lactamdurans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.20.1 | Fe2+ | required | Amycolatopsis lactamdurans | |
1.14.20.1 | Fe2+ | Km: 0.071 mM | Amycolatopsis lactamdurans | |
1.14.20.1 | Fe3+ | can replace Fe2+ under reducing conditions | Amycolatopsis lactamdurans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 27000 | - |
gel filtration | Amycolatopsis lactamdurans |
1.14.20.1 | 28000 | - |
1 * 28000, SDS-PAGE | Amycolatopsis lactamdurans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.1 | Amycolatopsis lactamdurans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.20.1 | - |
Amycolatopsis lactamdurans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | additional information | - |
- |
Amycolatopsis lactamdurans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.1 | additional information | no activity with isopenicillin N or 6-aminopenicillanic acid | Amycolatopsis lactamdurans | ? | - |
? | |
1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | no activity | Amycolatopsis lactamdurans | phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O | - |
? | |
1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | oxoglutarate analogs are not used as substrate | Amycolatopsis lactamdurans | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.20.1 | monomer | 1 * 28000, SDS-PAGE | Amycolatopsis lactamdurans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 25 | 30 | - |
Amycolatopsis lactamdurans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 5 | 40 | 20°C: 15% of maximal activity, 35°C: 15% of maximal activity | Amycolatopsis lactamdurans |
1.14.20.1 | 20 | 35 | 20°C: 90% of maximal activity, 35°C: 90% of maximal activity | Amycolatopsis lactamdurans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 25 | - |
60 min, stable | Amycolatopsis lactamdurans |
1.14.20.1 | 30 | - |
60 min, 25% loss of activity | Amycolatopsis lactamdurans |
1.14.20.1 | 65 | - |
60 min, complete inactivation | Amycolatopsis lactamdurans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 5 | 11 | - |
Amycolatopsis lactamdurans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 7 | - |
4°C, 120 h, 80% loss of activity | Amycolatopsis lactamdurans |
1.14.20.1 | 8 | - |
4°C, 120 h, 70% loss of activity | Amycolatopsis lactamdurans |
1.14.20.1 | 9 | - |
4°C, 120 h, 35% loss of activity | Amycolatopsis lactamdurans |