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Literature summary extracted from

  • Powers-Lee, S.G.
    N-Acetylglutamate synthase (1985), Methods Enzymol., 113, 27-35.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.1 arginine
-
 Homo sapiens
2.3.1.1 arginine 0.02 mM, 4.2fold activation Rattus norvegicus
2.3.1.1 Cationic polypeptides 4fold activation Rattus norvegicus
2.3.1.1 Triton X-100 0.1%, 4fold activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.1 Triton X-100, 0.1%, stabilizes liver enzyme Rattus norvegicus
2.3.1.1 use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.1 use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Escherichia coli
2.3.1.1 use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Homo sapiens
2.3.1.1 use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Rattus norvegicus
2.3.1.1 use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.1 coenzyme A 2.5 mM, 50% inhibition Escherichia coli
2.3.1.1 coenzyme A 1 mM, 20% inhibition Rattus norvegicus
2.3.1.1 high ionic strength
-
 Escherichia coli
2.3.1.1 high ionic strength
-
 Rattus norvegicus
2.3.1.1 N-acetylglutamate
-
 Escherichia coli
2.3.1.1 N-acetylglutamate 2 mM, 88% inhibition Rattus norvegicus
2.3.1.1 propionyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.1 0.76
-
 acetyl-CoA
-
 Rattus norvegicus
2.3.1.1 3.7
-
 L-glutamate
-
 Rattus norvegicus
2.3.1.1 4.6
-
 acetyl-CoA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.1 4.7
-
 L-glutamate partially purified enzyme Homo sapiens
2.3.1.1 8.1
-
 acetyl-CoA partially purified enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.1 57000
-
 3 * 57000, SDS-PAGE Rattus norvegicus
2.3.1.1 160000
-
 gel filtration, sucrose density gradient centrifugation Rattus norvegicus
2.3.1.1 190000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate Rattus norvegicus
-
 CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate Salmonella enterica subsp. enterica serovar Typhimurium enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate Escherichia coli enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate Pseudomonas aeruginosa enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Escherichia coli
-
-
-
2.3.1.1 Homo sapiens
-
-
-
2.3.1.1 Pseudomonas aeruginosa
-
-
-
2.3.1.1 Rattus norvegicus
-
-
-
2.3.1.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.1 liver
-
 Homo sapiens
-
2.3.1.1 liver
-
 Rattus norvegicus
-
2.3.1.1 small intestine
-
 Homo sapiens
-
2.3.1.1 small intestine mucosa Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 0.000014
-
-
 Rattus norvegicus
2.3.1.1 0.00013
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.1 0.00013
-
-
 Escherichia coli
2.3.1.1 0.000268
-
 activity in liver of adult Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + DL-2-aminopimelate
-
 Escherichia coli CoA + 2-acetylaminoheptanedioate
-
 ?
2.3.1.1 acetyl-CoA + DL-2-aminopimelate 5.2% of activity with L-glutamate Rattus norvegicus CoA + 2-acetylaminoheptanedioate
-
 ?
2.3.1.1 acetyl-CoA + glycine 2.9% of activity with L-glutamate Rattus norvegicus CoA + acetylaminoacetate
-
 ?
2.3.1.1 acetyl-CoA + L-2-aminoadipate
-
 Rattus norvegicus CoA + 2-acetylaminohexanedioate
-
 ?
2.3.1.1 acetyl-CoA + L-2-aminoadipate 1.3% of activity with L-glutamate Escherichia coli CoA + 2-acetylaminohexanedioate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Escherichia coli CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Homo sapiens CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Escherichia coli CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamine 5% of activity with acetyl-CoA Escherichia coli ?
-
 ?
2.3.1.1 acetyl-CoA + L-glutamine 6.8% of activity with L-glutamate Rattus norvegicus ?
-
 ?
2.3.1.1 propionyl-CoA + L-glutamate 4% of activity with acetyl-CoA Escherichia coli CoA + N-propionyl-L-glutamate
-
 ?
2.3.1.1 propionyl-CoA + L-glutamate 4.3% of activity with acetyl-CoA Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.1 trimer 3 * 57000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.1 8
-
 liver enzyme, in the absence of arginine Rattus norvegicus
2.3.1.1 8.5
-
-
 Rattus norvegicus
2.3.1.1 8.5
-
 liver enzyme, in the presence of arginine Homo sapiens
2.3.1.1 10
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.1 7
-
 highest stability Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.1 0.71
-
 propionyl-CoA
-
 Rattus norvegicus