EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.74 | wild type enzyme and HvCHS2 with different substrate requirements | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.74 | 0.001 | - |
feruloyl-CoA | - |
Hordeum vulgare | |
2.3.1.74 | 0.002 | - |
4-coumaroyl-CoA | - |
Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.74 | 43000 | - |
wild type enzyme migrates at 43000 Da, HvCHS2 migrated at a slightly lower molecular mass | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.74 | Hordeum vulgare | - |
barley, inoculated with fungus blumeria graminis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.74 | caffeoyl-CoA + 3 malonyl-CoA | wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate | Hordeum vulgare | eriodictyol + 4 CoA + 3 CO2 | - |
? | |
2.3.1.74 | cinnamoyl-CoA + malonyl-CoA | wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate | Hordeum vulgare | 5,7-dihydroxyflavanone + CoA + CO2 | - |
? | |
2.3.1.74 | ferulyl-CoA + malonyl-CoA | wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate | Hordeum vulgare | homoeriodictyol + CoA + CO2 | - |
? | |
2.3.1.74 | malonyl-CoA + 4-coumaroyl-CoA | wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate | Hordeum vulgare | naringenin chalcone + CoA + CO2 | - |
? |