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Literature summary extracted from

  • Zimowski, J.; Wojciechowski, Z.A.
    Partial purification and specificity of triacylglycerol: sterol acyltransferase from Sinapsis alba (1981), Phytochemistry, 20, 1799-1803.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.77 purification has to be carried out at 0-4°C Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.77 additional information reagents containing free sulfhydryl groups e.g. DTT have no effect Sinapis alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.77 cell membrane
-
 Sinapis alba
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.77 additional information divalent metal ions have no stimulatory effect Sinapis alba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.77 additional information
-
 two peaks, one more than 100000 Da, the other 18000 Da: the fraction with higher molecular weight may consist of the aggregated form of the enzyme of 18000 Da Sinapis alba
2.3.1.77 18000
-
 gel filtration Sinapis alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.77 additional information Sinapis alba the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.77 Sinapis alba
-
 white mustard
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.77 partial Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.77 root of seedling Sinapis alba
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.77 4°C, solubilized enzyme preparation, 24 h, 10% loss of activity Sinapis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.77 additional information enzyme has an absolute requirement for a beta-configuration of the hydroxyl group at C-3, rate of esterification is higher for sterols containing a planar ring system, triacylglycerols with unsaturated fatty acids are used more efficiently than fatty acids saturated with equal chain length, triacylglycerols with fatty acids C6-C22 can be used as acyl donors Sinapis alba ?
-
 ?
2.3.1.77 additional information the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain Sinapis alba ?
-
 ?
2.3.1.77 triacylglycerol + cholest-5-en-3beta-ol triacylglycerol from olive oil Sinapis alba diacylglycerols + cholesteryl ester
-
 ?
2.3.1.77 trilinoleoylglycerol + cholest-5-en-3beta-ol
-
 Sinapis alba dilinoleoylglycerol + cholesteryl linoleate
-
 ?
2.3.1.77 trioleoylglycerol + cholest-5-en-3beta-ol
-
 Sinapis alba dioleoylglycerol + cholesteryl oleate
-
 ?
2.3.1.77 tripalmitoylglycerol + 24R-methylcholest-5-en-3beta-ol i.e. campestero l Sinapis alba ?
-
 ?
2.3.1.77 tripalmitoylglycerol + 5alpha-androstan-3beta-ol i.e. androstanol Sinapis alba ?
-
 ?
2.3.1.77 tripalmitoylglycerol + 5alpha-cholestan-3beta-ol i.e. cholestanol, highest rate of esterification Sinapis alba ?
-
 ?
2.3.1.77 tripalmitoylglycerol + 5beta-cholestan-3beta-ol i.e. coprostanol Sinapis alba ?
-
 ?
2.3.1.77 tripalmitoylglycerol + stigmast-5-en-3beta-ol i.e. sitosterol Sinapis alba ?
-
 ?
2.3.1.77 tripalmitoylglycerol + stigmasta-5,22-dien-3beta-ol i.e. stigmasterol Sinapis alba ?
-
 ?
2.3.1.77 tristearoylglycerol + cholest-5-en-3beta-ol
-
 Sinapis alba distearoylglycerol + cholesteryl stearate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.77 52
-
-
 Sinapis alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.77 5.8
-
 assay at Sinapis alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.77 5 6.8
-
 Sinapis alba