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Literature summary extracted from

  • Jaklitsch, W.M.; Kubicek, C.P.
    Homocitrate synthase from Penicillium chrysogenum (1990), Biochem. J., 269, 247-253.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.3.14 enzyme is very unstable in crude and protamine sulphate-treated desalted extracts Penicillium chrysogenum
2.3.3.14 freezing and thawing results in 50% loss of activity Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 AMP 1 mM, 13-15% inhibition Penicillium chrysogenum
2.3.3.14 CoA
-
 Penicillium chrysogenum
2.3.3.14 EDTA 5 mM, 30% inhibition Penicillium chrysogenum
2.3.3.14 L-Lys 50% inhibition by 0.053 mM, at 6 mM 2-oxoglutarate Penicillium chrysogenum
2.3.3.14 Mg2+ 10 mM MgSO4, 30% inhibition Penicillium chrysogenum
2.3.3.14 Mn2+ 0.025 mM, 35% inhibition Penicillium chrysogenum
2.3.3.14 Zn2+ 0.025 mM, 60% inhibition Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.14 2.2
-
 2-oxoglutarate
-
 Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.14 cytosol main activity located in Penicillium chrysogenum 5829
-
2.3.3.14 mitochondrion 25% of total activity Penicillium chrysogenum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.14 155000
-
 gel filtration Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.14
-
 Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.14 additional information
-
-
 Penicillium chrysogenum

Storage Stability

EC Number Storage Stability Organism
2.3.3.14 4-6°C, enzyme in cell-free extract in presence of 20% glycerol, 65% loss of activity after 48 h Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Penicillium chrysogenum 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.14 7.5 8
-
 Penicillium chrysogenum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.14 0.008
-
 L-Lys
-
 Penicillium chrysogenum