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Literature summary extracted from

  • Bergeron, L.J.; Burne, R.A.
    Roles of fructosyltransferase and levanase-sucrase of Actinomyces naeslundii in fructan and sucrose metabolism (2001), Infect. Immun., 69, 5395-5402.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Actinomyces naeslundii strain Lev1 Actinomyces naeslundii
2.4.1.10 overexpression as His-tagged protein in Escherichia coli strain His-Is M15 and DH10B, partial gene ftf without the predicted signal sequence Actinomyces naeslundii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 additional information allelic mutagenesis of wild-type strain, inactivation of ftf gene, no remaining levansucrase activity in the mutated strain Actinomyces naeslundii
2.4.1.10 T14V levanase-deficient mutant Actinomyces naeslundii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 cell associated
-
 Actinomyces naeslundii
-
-
2.4.1.10 extracellular
-
 Actinomyces naeslundii
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Actinomyces naeslundii enzyme is probably involved in initiation and progression of dental caries and periodontal diseases glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Actinomyces naeslundii capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Actinomyces naeslundii WVU45 enzyme is probably involved in initiation and progression of dental caries and periodontal diseases glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Actinomyces naeslundii WVU45 capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Actinomyces naeslundii
-
-
-
2.4.1.10 Actinomyces naeslundii
-
 gene ftf
-
2.4.1.10 Actinomyces naeslundii WVU45
-
-
-
2.4.1.10 Actinomyces naeslundii WVU45
-
 gene ftf
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.10 no modification
-
 Actinomyces naeslundii

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 recombinant His-tagged enzyme from E. coli Actinomyces naeslundii
2.4.1.10 SDS-PAGE, immobilon-P membrane Actinomyces naeslundii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.10 culture supernatant
-
 Actinomyces naeslundii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 0.1217
-
 sucrose, in culture supernatants Actinomyces naeslundii
2.4.1.10 0.13
-
 glucose, in culture supernatants Actinomyces naeslundii
2.4.1.10 2.562
-
 glucose, in wild-type strain Actinomyces naeslundii
2.4.1.10 3.747
-
 sucrose, in wild-type strain Actinomyces naeslundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 inulin + H2O
-
 Actinomyces naeslundii ?
-
 ?
2.4.1.10 inulin + H2O
-
 Actinomyces naeslundii WVU45 ?
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n reverse reaction is probably catalyzed by a different levanase Actinomyces naeslundii glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n enzyme is probably involved in initiation and progression of dental caries and periodontal diseases Actinomyces naeslundii glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n reverse reaction is probably catalyzed by a different levanase Actinomyces naeslundii WVU45 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n enzyme is probably involved in initiation and progression of dental caries and periodontal diseases Actinomyces naeslundii WVU45 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Actinomyces naeslundii alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth Actinomyces naeslundii alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Actinomyces naeslundii WVU45 alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth Actinomyces naeslundii WVU45 alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 68000-72000 Actinomyces naeslundii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 levanase-sucrase
-
 Actinomyces naeslundii
2.4.1.10 LevJ
-
 Actinomyces naeslundii