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Literature summary extracted from
- Purification and properties of Bacillus coagulans cyclomaltodextrin glucanotransferase (1991), J. Ferment. Bioeng., 71, 322-328.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | - |
Geobacillus stearothermophilus |
| 2.4.1.19 | cloned and overexpressed in Bacillus subtilis | Paenibacillus macerans |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | - |
Geobacillus stearothermophilus |
| 2.4.1.19 | - |
Paenibacillus macerans |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.19 | not inactivated by 2 M guanidium chloride | Weizmannia coagulans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.19 | Ag+ | - |
Weizmannia coagulans |  |
| 2.4.1.19 | Al3+ | - |
Weizmannia coagulans | |
| 2.4.1.19 | chromate | - |
Weizmannia coagulans | |
| 2.4.1.19 | Cr3+ | - |
Weizmannia coagulans | |
| 2.4.1.19 | Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
| 2.4.1.19 | Cu2+ | - |
Weizmannia coagulans | |
| 2.4.1.19 | Hg2+ | - |
Weizmannia coagulans | |
| 2.4.1.19 | HgSO4 | - |
Weizmannia coagulans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.19 | 0.08 | - |
beta-cyclodextrin | cyclodextrin opening | Paenibacillus macerans | |
| 2.4.1.19 | 0.25 | - |
gamma-cyclodextrin | - |
Weizmannia coagulans | |
| 2.4.1.19 | 0.25 | - |
gamma-cyclodextrin | cyclodextrin opening | Paenibacillus macerans | |
| 2.4.1.19 | 0.51 | - |
beta-cyclodextrin | cyclodextrin opening | Weizmannia coagulans | |
| 2.4.1.19 | 0.59 | - |
alpha-cyclodextrin | coupling | Weizmannia coagulans | |
| 2.4.1.19 | 0.73 | - |
alpha-cyclodextrin | cyclodextrin opening | Weizmannia coagulans | |
| 2.4.1.19 | 0.88 | - |
gamma-cyclodextrin | cyclodextrin opening | Weizmannia coagulans | |
| 2.4.1.19 | 1.26 | - |
alpha-cyclodextrin | coupling | Paenibacillus macerans | |
| 2.4.1.19 | 2.32 | - |
alpha-cyclodextrin | cyclodextrin opening | Paenibacillus macerans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.19 | BaCl2 | relative activity 102.4% | Weizmannia coagulans | |
| 2.4.1.19 | Ca2+ | - |
Geobacillus stearothermophilus | |
| 2.4.1.19 | Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
| 2.4.1.19 | Ca2+ | - |
Niallia circulans | |
| 2.4.1.19 | Ca2+ | contains 2 mol Ca2+ per mol of enzyme, increases heat stability, denaturation temperature shifted from 70°C to 88°C | Weizmannia coagulans | |
| 2.4.1.19 | Ca2+ | contains 1 mol Ca2+ per mol of enzyme | Paenibacillus macerans | |
| 2.4.1.19 | CaCl2 | relative activity 106.7% | Weizmannia coagulans | |
| 2.4.1.19 | FeSO4 | relative activity 107.6% | Weizmannia coagulans | |
| 2.4.1.19 | Li2SO4 | relative activity 106% | Weizmannia coagulans | |
| 2.4.1.19 | MgSO4 | relative activity 103.2% | Weizmannia coagulans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 33000 | - |
gel filtration Superose 6 | Weizmannia coagulans |
| 2.4.1.19 | 34000 | - |
gel filtration BioGel P-100 | Weizmannia coagulans |
| 2.4.1.19 | 35000 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 35000 | - |
- |
Bacillus ohbensis |
| 2.4.1.19 | 35000 | - |
gel filtration Superose 6 | Paenibacillus macerans |
| 2.4.1.19 | 38000 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 38000 | - |
gel filtration BioGel P-100 | Paenibacillus macerans |
| 2.4.1.19 | 64000 | - |
- |
Bacillus subtilis |
| 2.4.1.19 | 65000 | - |
- |
Paenibacillus macerans |
| 2.4.1.19 | 65000 | - |
sedimentation equilibrium, SDS-PAGE | Weizmannia coagulans |
| 2.4.1.19 | 65500 | - |
amino acid composition | Weizmannia coagulans |
| 2.4.1.19 | 66000 | - |
- |
Priestia megaterium |
| 2.4.1.19 | 68000 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 68000 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 68000 | - |
sedimentation equilibrium, amino acid composition | Paenibacillus macerans |
| 2.4.1.19 | 85000 | 88000 | - |
Niallia circulans |
| 2.4.1.19 | 88000 | - |
- |
Niallia circulans |
| 2.4.1.19 | 200000 | - |
- |
Niallia circulans |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | sodium dodecyl sulfate | not inactivated by 0.2% | Weizmannia coagulans |
| 2.4.1.19 | urea | not inactivated by 8 M | Weizmannia coagulans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.19 | Bacillus ohbensis | - |
- |
- |
| 2.4.1.19 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 2.4.1.19 | Bacillus subtilis | - |
no.313 | - |
| 2.4.1.19 | Bacillus subtilis 313 | - |
no.313 | - |
| 2.4.1.19 | Geobacillus stearothermophilus | - |
TC-60 | - |
| 2.4.1.19 | Geobacillus stearothermophilus TC-60 | - |
TC-60 | - |
| 2.4.1.19 | Klebsiella oxytoca | - |
formerly Klebsiella pneumoniae M5a1 | - |
| 2.4.1.19 | Klebsiella pneumoniae | - |
- |
- |
| 2.4.1.19 | Niallia circulans | - |
B. sp. var. alkalophilus No. 38-2, ATCC 21783 | - |
| 2.4.1.19 | Paenibacillus macerans | - |
formerly Aeromonas macerans | - |
| 2.4.1.19 | Priestia megaterium | - |
- |
- |
| 2.4.1.19 | Weizmannia coagulans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | - |
Weizmannia coagulans |
| 2.4.1.19 | - |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | - |
Paenibacillus macerans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 0.01 | - |
gamma-cyclodextrin, opening | Paenibacillus macerans |
| 2.4.1.19 | 0.02 | - |
beta-cyclodextrin, opening | Paenibacillus macerans |
| 2.4.1.19 | 1.24 | - |
gamma-cyclodextrin, opening | Paenibacillus macerans |
| 2.4.1.19 | 1.42 | - |
alpha-cyclodextrin, opening | Paenibacillus macerans |
| 2.4.1.19 | 1.7 | - |
beta-cyclodextrin, opening | Weizmannia coagulans |
| 2.4.1.19 | 2.4 | - |
maltotriose, disproportionation | Paenibacillus macerans |
| 2.4.1.19 | 14.83 | - |
alpha-cyclodextrin, opening | Weizmannia coagulans |
| 2.4.1.19 | 15.5 | - |
maltotriose, disproportionation | Weizmannia coagulans |
| 2.4.1.19 | 37.4 | - |
alpha-cyclodextrin, coupling | Paenibacillus macerans |
| 2.4.1.19 | 203.1 | - |
alpha-cyclodextrin, coupling | Weizmannia coagulans |
| 2.4.1.19 | 883 | - |
starch, cyclodextrin synthesis | Weizmannia coagulans |
| 2.4.1.19 | 904 | - |
- |
Weizmannia coagulans |
| 2.4.1.19 | 1027 | - |
- |
Paenibacillus macerans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.19 | -20°C, 0.05 M Tris-HCl, pH 7.0 or sodium acetate buffer, pH 6.5 for at least 6 months without loss of activity | Weizmannia coagulans |
| 2.4.1.19 | 4°C, pH 5.0-10.0, does not lose activity for 1 month | Weizmannia coagulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | beta-cyclodextrin + glycosyl acceptor | - |
Geobacillus stearothermophilus | alpha-cyclodextrin + maltooligosaccharide | - |
r | |
| 2.4.1.19 | beta-cyclodextrin + glycosyl acceptor | - |
Weizmannia coagulans | alpha-cyclodextrin + maltooligosaccharide | - |
r | |
| 2.4.1.19 | beta-cyclodextrin + glycosyl acceptor | - |
Paenibacillus macerans | alpha-cyclodextrin + maltooligosaccharide | - |
r | |
| 2.4.1.19 | beta-cyclodextrin + glycosyl acceptor | - |
Geobacillus stearothermophilus TC-60 | alpha-cyclodextrin + maltooligosaccharide | - |
r | |
| 2.4.1.19 | maltotriose + maltotetraose | - |
Weizmannia coagulans | maltopentaose | - |
r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | strain ATCC 21783, pH 4.5-4.7, producing ratio 23.5: 1.0: 1.0, pH 7.0 0.2: 6.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Priestia megaterium | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | product ratio 2.0:5.0:1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | product ratio 2.0:5.0:1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus subtilis | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 0.0: 0.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 5.5: 8.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus ohbensis | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 0.0: 4.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | potato starch, sweet potato starch, rice starch, corn starch, wheat starch | Paenibacillus macerans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 5.0: 2.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | potato starch, sweet potato starch, rice starch, corn starch, wheat starch | Weizmannia coagulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | potato starch, 20 h at 50°C, ratio 8.1: 8.9: 1.0, various conditions, product proportions 1.0: 1.0: 0.3 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus TC-60 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 5.5: 8.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus subtilis 313 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | producing ratio 0.0: 0.0: 1.0 | r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus subtilis | gamma-cyclodextrin | - |
r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | gamma-cyclodextrin | - |
r | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | gamma-cyclodextrin | - |
? | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Bacillus subtilis 313 | gamma-cyclodextrin | - |
r | |
| 2.4.1.19 | soluble starch + sucrose | - |
Weizmannia coagulans | maltosylfructose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | monomer | - |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | monomer | - |
Niallia circulans |
| 2.4.1.19 | monomer | - |
Paenibacillus macerans |
| 2.4.1.19 | monomer | 1 * 65000, SDS-PAGE | Weizmannia coagulans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | C-CGTase | - |
Weizmannia coagulans |
| 2.4.1.19 | M-CGTase | - |
Paenibacillus macerans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 45 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 45 | - |
- |
Niallia circulans |
| 2.4.1.19 | 50 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 50 | - |
- |
Niallia circulans |
| 2.4.1.19 | 55 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 55 | - |
- |
Priestia megaterium |
| 2.4.1.19 | 55 | - |
- |
Weizmannia coagulans |
| 2.4.1.19 | 55 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 55 | - |
- |
Niallia circulans |
| 2.4.1.19 | 55 | - |
- |
Paenibacillus macerans |
| 2.4.1.19 | 55 | - |
- |
Bacillus ohbensis |
| 2.4.1.19 | 60 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 60 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 60 | - |
- |
Niallia circulans |
| 2.4.1.19 | 60 | - |
- |
Paenibacillus macerans |
| 2.4.1.19 | 60 | - |
- |
Bacillus ohbensis |
| 2.4.1.19 | 65 | - |
- |
Bacillus subtilis |
| 2.4.1.19 | 65 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 70 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 70 | - |
- |
Weizmannia coagulans |
| 2.4.1.19 | 70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 30 | 70 | - |
Weizmannia coagulans |
| 2.4.1.19 | 30 | 70 | - |
Niallia circulans |
| 2.4.1.19 | 30 | 70 | - |
Paenibacillus macerans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 70 | 80 | does not lose activity by heat treatment for 10 min at 70-75°C, pH 5.5-9.5 in presence of CaCl2, loses half of the activity by heating at 80°C for 10 min | Weizmannia coagulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 4.5 | 4.7 | - |
Niallia circulans |
| 2.4.1.19 | 5 | 5.7 | - |
Priestia megaterium |
| 2.4.1.19 | 5 | 5.7 | - |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 5 | 5.7 | - |
Paenibacillus macerans |
| 2.4.1.19 | 5.5 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 5.5 | - |
- |
Niallia circulans |
| 2.4.1.19 | 5.5 | - |
- |
Paenibacillus macerans |
| 2.4.1.19 | 5.5 | - |
- |
Bacillus ohbensis |
| 2.4.1.19 | 6 | - |
- |
Geobacillus stearothermophilus |
| 2.4.1.19 | 6 | - |
- |
Weizmannia coagulans |
| 2.4.1.19 | 6 | - |
- |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 6 | - |
- |
Niallia circulans |
| 2.4.1.19 | 7 | - |
- |
Priestia megaterium |
| 2.4.1.19 | 7 | - |
- |
Niallia circulans |
| 2.4.1.19 | 7 | - |
- |
Paenibacillus macerans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 5 | 10.5 | activity is not lost by incubation at 4°C for 1 month | Weizmannia coagulans |
| 2.4.1.19 | 5.5 | 9.5 | stable at 70°C | Weizmannia coagulans |
| 2.4.1.19 | 6 | 9 | stable at 70°C | Niallia circulans |
| 2.4.1.19 | 6 | 8 | - |
Niallia circulans |
| 2.4.1.19 | 6 | 8 | stable at 50°C | Bacillus subtilis |
| 2.4.1.19 | 6 | 10 | - |
Weizmannia coagulans |
| 2.4.1.19 | 6 | 10 | stable at 65°C | Niallia circulans |
| 2.4.1.19 | 6 | 9.5 | stable at 55°C | Niallia circulans |
| 2.4.1.19 | 6.5 | 8.5 | stable at 50°C | Paenibacillus macerans |
| 2.4.1.19 | 6.5 | 9.5 | stable at 55°C | Bacillus ohbensis |
| 2.4.1.19 | 7 | 10 | - |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | 7 | 10 | stable at 65°C | Priestia megaterium |
| 2.4.1.19 | 7 | 9.2 | stable at 50°C | Geobacillus stearothermophilus |
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