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Literature summary extracted from

  • Aragon, J.J.; Lowenstein, J.M.
    A survey of enzymes which generate or use acetoacetyl thioesters in rat liver (1983), J. Biol. Chem., 258, 4725-4733.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.11 cysteine stimulation Rattus norvegicus
3.1.2.11 dithiothreitol stimulation Rattus norvegicus
3.1.2.11 glutathione stimulation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.11 0.021
-
 acetoacetyl-CoA liver cytosol, absence of glutathione Rattus norvegicus
3.1.2.11 0.025
-
 acetoacetyl-CoA liver cytosol, presence of glutathione Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.11 cytoplasm
-
 Rattus norvegicus 5737
-
3.1.2.11 mitochondrion
-
 Rattus norvegicus 5739
-
4.1.3.4 cytoplasm 13% of activity Rattus norvegicus 5737
-
4.1.3.4 mitochondrion 87% of activity Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA Rattus norvegicus acetoacetate as fuel for extrahepatic tissues, involved in mevalonate synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.11 Rattus norvegicus
-
-
-
4.1.3.4 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.11 liver
-
 Rattus norvegicus
-
4.1.3.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.11 acetoacetyl-CoA + H2O
-
 Rattus norvegicus acetoacetate + CoA
-
 ?
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA acetoacetate as fuel for extrahepatic tissues, involved in mevalonate synthesis Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 7.4
-
 enzyme assay at Rattus norvegicus